Anti-fatigue compound preparation, and preparation method and anti-fatigue application thereof
A compound preparation and anti-fatigue technology, which is applied in the field of biotechnology medicinal and edible plants, can solve the problems of slow onset of action, and achieve the effect of increasing reserves, large health care and clinical application value, and small dosage
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[0059] 1 Materials and methods
[0060] 1.1 Sample:
[0061] 1.2 Experimental animals: five-month-old middle-aged male ICR mice; eight-month-old old male C57 mice.
[0062] 1.3 Instruments and reagents: swimming box, lead wire, full-wavelength microplate reader, constant temperature water bath, centrifuge, liver
[0063] Glycogen Detection Kit (Nanjing Jiancheng, Cat. No. A043), Lactic Acid Detection Kit (Nanjing Jiancheng, Cat. No. A019-1), Urea Nitrogen Kit (Nanjing Jiancheng, Cat. A001-1), MDA detection kit (Nanjing Jiancheng, article number A003-1).
[0064] 1.4 Experimental method:
[0065] A. The first batch: 60 male five-month-old middle-aged ICR mice were randomly divided into 4 groups, 15 in each group. The low-dose group received 100 mg / kg intraperitoneal injection, the middle-dose 300 mg / kg intraperitoneal injection, and the high-dose 900 mg / kg intraperitoneal injection. The control group was given the same volume of normal saline. Administer once a day from 1...
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