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A biomarker for esophageal cancer and its application

A biomarker, esophageal cancer technology, applied in the field of esophageal cancer biomarkers

Active Publication Date: 2020-08-07
JINAN UNIVERSITY +1
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

However, there are few studies on mesoderm-specific transcripts in tumors, especially in esophageal carcinoma

Method used

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  • A biomarker for esophageal cancer and its application
  • A biomarker for esophageal cancer and its application
  • A biomarker for esophageal cancer and its application

Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0050] collect samples

[0051]Collect 100 cases of surgically resected esophageal cancer specimens and 80 cases of normal tissues, as well as 40 cases of primary esophageal cancer metastasis tissues purchased from Shanghai Xinchao Biotechnology Co., Ltd.

[0052] tissue microarray

[0053] Tissue microarray (TMA) (Biomax, Rockville, MD) immunohistochemical method was used to analyze the expression of MEST on 100 cases of esophageal cancer tissues and 80 cases of normal tissues to determine the correlation between the upregulation of MEST expression and the occurrence and development of esophageal cancer and perform MEST immunohistochemical analysis on another tissue microarray (TMA) of 40 primary esophageal cancer metastatic tissues purchased by Shanghai Xinchao Biotechnology Co., Ltd. to compare the expression of MEST in primary lesions and metastatic lesions Then, the correlation between MEST expression level and tumor metastasis was determined.

[0054] Immunohistochemis...

Embodiment 2

[0057] collect samples

[0058] Sera from 100 patients and 100 healthy controls were collected from Jinan University and Shantou University.

[0059] ELISA detection

[0060] The expression of MEST in the serum of 100 patients with esophageal cancer and 100 healthy controls was detected by enzyme-linked immunosorbent assay (ELISA) to determine the correlation between the upregulation of MEST expression and esophageal squamous cell carcinoma.

Embodiment 3

[0061] Embodiment 3 result analysis

[0062] 1. MEST expression

[0063] In order to study the clinical significance of MEST in human tumors, the expression level of MEST was detected by immunohistochemistry, and the expression level of MEST in 100 cases of esophageal cancer tissues and 80 cases of normal tissues was analyzed by tissue microarray method. The dyeing results are scored, and the scoring rules are as follows: figure 1 As shown, the staining intensity of TMA chip scanning: divided into four grades (1-4), 1 point represents negative, 2 points represent weak positive, 3 points represent medium positive, 4 points represent strong positive. Two pathological methods were used to test and independently score the immunostaining degree of the sections. Samples with a score of 1 to 2 were considered low expression, and samples with a score of 3 to 4 were considered high expression; normal tissue and esophageal cancer tissue The staining results and analysis charts are sho...

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Abstract

The invention discloses a biomarker for the esophagus cancer and an application of the biomarker. The biomarker is a mesoderm specific transcription factor, the amino acid sequence of the biomarker isshown in SEQ ID NO:1, the biomarker used for diagnosis of metastasis of the esophagus cancer as well as animation prognosis, the mesoderm specific transcription factor is used to prepare an agent and / or medicine for assisted diagnosis of the esophagus cancer, evaluation of the therapeutic effect and monitoring of metastasis and relapse, has an obvious effect, and is good in specificity.

Description

technical field [0001] The invention relates to the field of biotechnology, in particular to a biomarker of esophageal cancer and its application. Background technique [0002] Esophageal cancer is one of the cancers with a high mortality rate in the world, and the 5-year survival rate is less than 20%. In China, esophageal cancer is mainly manifested as esophageal squamous cell carcinoma (ESCC). Although the development of clinical technology has improved the survival rate of some patients with esophageal squamous cell carcinoma, the high mortality rate of esophageal cancer is mainly attributed to tumor metastasis. Tumor metastasis includes a series of processes such as cancer cell invasion, angiogenesis, spread and proliferation in specific organs and tissues, and is the most lethal feature of cancer, accounting for more than 90% of cancer-related deaths. For example, in the "seed and soil" hypothesis of cancer cell metastasis proposed by Paget, the intrinsic characterist...

Claims

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Application Information

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Patent Type & Authority Patents(China)
IPC IPC(8): G01N33/68G01N33/574
CPCG01N33/57407G01N33/57484G01N33/68
Inventor 许雯雯李斌李军旗廖化新
Owner JINAN UNIVERSITY
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