Primer ACY of Ty-3 molecular marker and indexing method thereof

Abstract

The invention relates to a primer ACY of a Ty-3 molecular marker and an indexing method thereof. Genome DNA of tomato leaves of two anti-infection materials of a tomato yellow leaf curl virus is subjected to PCR amplification, so that an accurate and efficient molecular marker for distinguishing a disease-resistant variety from a susceptible variety is obtained, and the marker is stable and reliable, and therefore, tomato marker-assistant selective breeding is facilitated. The special Ty-3 molecular marker amplifies a 132bp stripe in the disease-resistant variety and amplifies a 123bp stripe in the susceptible variety, and the stripes are clear; and a primer is designed in a gene region, so that false positive generated by exchange is not generated, and accurate reliability of identification results is guaranteed; and the identification results are completely consistent with a field phenotype of a used material.

Description

technical field [0001] The invention relates to a Ty-3 molecularly marked primer ACY and an indexing method thereof, belonging to the technical field of bioengineering. Background technique [0002] Tomato yellow leaf curl disease (TYLCD) is caused by tomato yellow leaf curl virus (TYLCV) in the Geminiviridae family devastating disease. TYLCD was first reported in Israel in the late 1930s, and in the 1960s, the disease broke out on tomatoes in the Mediterranean Basin (Antignus et al. 1994), and subsequently spread to Africa, Europe, Asia, the Caribbean, and North America. , the occurrence of this disease often causes huge yield losses in tomato, up to 100% (Antignus et al. 1994; Pico et al. 1996; Czosnek et al. 1997; Polston et al. 1997; Delatte et al. 2005). Chinese tomato yellow leaf curl disease was first discovered in Nanning, Guangxi in 1998 (Liu Yule et al., 1998), and then TYLCD also occurred in different tomato growing areas in China. For a long time, TYLCD has be...

AI Technical Summary

Problems solved by technology

However, there is a certain distance between most molecular markers and resistance genes, and false positives may occur due to the exchange of linked markers and resistance genes during the marking process (Ji et al., 2007, 2012)

In addition, some markers, such as CAPS markers, require two lengthy steps of PCR amplification and restriction endonuclease digestion of the amplified products in practical applications, which makes their genotyping time-consuming and expensive

Images