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Combined enzyme for efficiently digesting goat testicular tissue

A technology of combining enzymes and goats, applied in the directions of glycosylase, tissue culture, enzymes, etc., to achieve the effect of high proportion, good use effect and simple formula

Inactive Publication Date: 2018-03-02
泊迈生物医学检测(苏州)有限公司
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  • Abstract
  • Description
  • Claims
  • Application Information

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Problems solved by technology

Germ stem cells have been shown to have pluripotency similar to embryonic stem cells, but there is still no stable isolation method for single cells from goat testis tissue

Method used

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  • Combined enzyme for efficiently digesting goat testicular tissue
  • Combined enzyme for efficiently digesting goat testicular tissue
  • Combined enzyme for efficiently digesting goat testicular tissue

Examples

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Embodiment 1

[0019] In this example, a combined enzyme for efficient digestion of goat testicular tissue was used, including 2 mg / mL collagenase, 20 μg / mL deoxyribonuclease, 2 mg / mL neutral protease and 0.1 mg / mL ethylenediaminetetraacetic acid.

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Abstract

The invention discloses combined enzyme for efficiently digesting goat testicular tissue. The combined enzyme comprises collagenase, deoxyribonuclease, neutral protease, and ethylene diamine tetraacetic acid. The combined enzyme has the advantages of simple formula and good usage effect, and can obtain single cell from the goat testicular tissue in a stable mode, the cell survival rate and proportion of germline stem cell in the obtained cell are high, and the after culture, the typical germline stem cell clone appears.

Description

technical field [0001] The invention relates to the field of biotechnology, in particular to a combined enzyme for efficiently digesting goat testicular tissue. Background technique [0002] With the continuous development and progress of modern biotechnology, new disease treatment methods and methods are constantly attracting public attention. Among them, stem cell therapy is currently considered to be the most potential and most promising treatment method. However, due to the limitations of existing stem cells in terms of their differentiation potential, ethical and moral restrictions, and safety, the use of stem cells for cell therapy is subject to increasingly strict supervision. The development of a new resource for cell therapy is the most urgent need for current stem cell development. Germ stem cells have been proven to have pluripotency similar to embryonic stem cells, but there is still no stable isolation method for single cells from goat testis tissue. Therefor...

Claims

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Application Information

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IPC IPC(8): C12N9/50C12N9/22C12N9/76C12N9/26C12N5/071
CPCC12N5/0683C12N9/22C12N9/2474C12N9/50C12N9/6408C12N2509/00C12Y302/01035C12Y304/21004
Inventor 孙振华曹晖
Owner 泊迈生物医学检测(苏州)有限公司
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