Method for effectively improving yield of mycelia in phellinus igniarius liquid-state culture

Abstract

The invention relates to an effective method for improving yield of mycelia in phellinus igniarius liquid-state culture. The following raw materials are adopted: corn starch, soybean meal, low-molecular-weight chitosan oligosaccharide and inorganic salt. Firstly, the corn starch and the soybean meal are separately subjected to enzymolysis by virtue of neutral protease, amylase and cellulase undera liquid condition, so that a treatment solution is prepared. And then, a culture medium is prepared from the enzymolysis treatment solution, the chitosan oligosaccharide and the inorganic salt, the culture medium is inoculated with phellinus igniarius seeds, culture conditions are controlled, and liquid fermentation is conducted in a fed-batch mode, so that the phellinus igniarius mycelia can beobtained. The method comprises steps of raw material enzymolysis pre-treatment, culture medium preparing, fermentation culture and the like. The method provided by the invention is easy to operate, stable in process, high in yield of the mycelia, low in production cost and easy for industrialization and marketization.

Description

technical field [0001] The invention relates to a liquid fermentation culture method for effectively improving the output of Phellinus mycelium, belonging to the field of microbial engineering. Background technique [0002] Phellinus is a large rare medicinal fungus, which mainly has anti-tumor, liver-protecting and anti-liver, anti-mutation and mutation pharmacology, and can be used to treat blood metrorrhagia, blood stranguria, proctoptosis and diarrhea, leukorrhagia, amenorrhea, spleen deficiency and diarrhea , hypoglycemic and other effects. It is expensive and in short supply in the international pharmaceutical market. Along with the increasing consumption of Phellinus Phellinus, wild resources are acquired in a predatory manner in various producing areas, and the natural and wild Phellinus Phellinus resources are becoming less and less. Due to the particularity and complexity of the physiological state and the restriction of the environment, Phellinus Phellinus needs...

AI Technical Summary

Problems solved by technology

At present, the artificial liquid culture research is mainly to explore the medium components and growth conditions of Phellinus mycelium growth, and determine the culture technology, but there are some deficiencies or disadvantages, such as large inoculum amount, large scale of seed culture, 10%, Even 15%-20%, larger-scale cultivation is limited by the amount of seeds, resulting in high cost; in addition, the cultivation cycle is long, 6-7 days, even up to 10 days, which increases the risk of bacterial contamination, and water, electricity, steam and other energy Increased consumption