Novel design of enzyme-linked immunsorbent assay plates and systems and methods of use thereof

An enzyme-linked immunosorbent (ELISA) and bottom plate technology, applied in the field of molecular biology, can solve problems such as high maintenance costs and the requirement of uninterrupted power supply

Inactive Publication Date: 2018-02-16
AGENCY FOR SCI TECH & RES
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  • Claims
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Problems solved by technology

However, their implementation in resource-constrained settings is severely limited by high maintenance costs and the need for sophisticated instrumentation, trained personnel, and uninterrupted power supply

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  • Novel design of enzyme-linked immunsorbent assay plates and systems and methods of use thereof
  • Novel design of enzyme-linked immunsorbent assay plates and systems and methods of use thereof
  • Novel design of enzyme-linked immunsorbent assay plates and systems and methods of use thereof

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Embodiment Construction

[0046] The present disclosure provides a novel plate design for performing ELISAs that can be used in a miniaturized format for simple ultra-rapid detection such as IgG responses against multiple antigens or detection of cytokines in supernatants such as IFN-γ. This concept of detecting the presence of immune molecules in body fluids of a subject is then used to diagnose and identify specific diseases such as tuberculosis.

[0047] In one instance, despite the identification of M. tuberculosis as the cause of tuberculosis more than a century ago, diagnosing tuberculosis remains a major challenge in resource-limited settings. Indeed, the same can be said, for example, for bacterial infections caused by similar bacteria or other infectious agents causing diseases or conditions with unclear symptoms. For example for the vast majority of patients in endemic countries eg tuberculosis diagnosis mainly depends on sputum microscopy and culture which has many limitations in sensitivit...

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Abstract

The present disclosure refers to an enzyme-linked immunosorbent assay (ELISA) plate comprising at least one row of reaction chambers, wherein the reaction chambers in the same row are in fluid communication with each other. Also enclosed is a system for detecting one or more target analytes comprising an ELISA plate as described herein, a plurality of magnetic beads and a magnet configured to cooperate with the magnetic beads. Also encompassed is a method of performing an ELISA assay which comprises of moving magnetic beads through subsequent reaction chambers, wherein the reaction chambers are alternatingly filled with a non-aqueous liquid, such as silicone oil, and aqueous ELISA reagents.

Description

[0001] Cross References to Related Applications [0002] This application claims priority to Singapore Provisional Application No. 10201503435Q filed 30 April 2015, the contents of which are hereby incorporated by reference in their entirety for all purposes. field of invention [0003] The present invention relates generally to the field of molecular biology. In particular, the present invention relates to the use of specially designed plates for the detection of analytes. Background of the invention [0004] The management and control of many diseases (eg, tuberculosis, TB) remain a major threat to public health, partly due to the lack of cost-effective, sensitive and rapid diagnostic tests. For example, for tuberculosis, sputum smear microscopy is currently the most commonly used point-of-care (POC) method for diagnosis in endemic countries, despite its poor sensitivity (30-60%). Although "gold standard" bacterial culture does provide the required sensitivity (>90%),...

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Application Information

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Patent Type & Authority Applications(China)
IPC IPC(8): G01N33/53
CPCG01N33/5304B01L3/5025G01N33/6854B01L2300/0829B01L2300/0858B01L2400/043B01L2200/0668B01L2300/087B01L2300/161B01L3/5023B01L2300/08B01L2300/0877G01N33/54386G01N33/5695G01N33/6863G01N33/6866G01N33/6869G01N2333/35G01N2333/525G01N2333/55G01N2333/57G01N2800/60
Inventor V·摩尼A·辛格哈尔G·德利贝罗
Owner AGENCY FOR SCI TECH & RES
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