Pigeon ttv and pigeon new adenovirus double Evagreen real-time fluorescent quantitative PCR detection kit

Abstract

The invention provides a double EvaGreen real-time fluorescent quantitative PCR detection kit for pigeon TTV and new pigeon adenovirus, the kit includes primers whose sequence is shown in SEQ ID NO.1‑4, and has high specificity and sensitivity. The established method can simultaneously detect pigeon TTV and pigeon new adenovirus infection in pigeon flocks, which simplifies operating procedures and saves costs. After the real-time fluorescent quantitative PCR reaction is completed, the result can be directly judged by observing the peak value of the melting curve (Tm value). The establishment of the present invention can fill in the gaps in related fields at home and abroad.

Description

technical field [0001] The invention provides a double EvaGreen real-time fluorescent quantitative PCR detection kit for pigeon TTV and new pigeon adenovirus, which belongs to the field of animal infectious diseases. Background technique [0002] Transfusion-transmitted virus, also known as Torque teno virus (TTV), according to the latest classification of the International Committee on Taxonomy of Viruses (ICTV), it is classified as Anelloviridae, Alphatorquevirus ). TTV is a kind of non-enveloped, single-stranded circular DNA virus. Studies have found that the length of the genome of TTV virus varies from species to species. The genome of human TTV is 3.2kb-3.9kb, the genome of pig and dog is about 2.8kb, and the genome of feline TTV is about 2.1kb. The analysis of TTV found that the TTV genome consists of coding regions and non-coding regions. It is generally believed that TTV contains two large open reading frames (ORFs): ORF1 and ORF2, which encode replication-associ...

AI Technical Summary

Problems solved by technology

However, there are currently no primers research reports based on the saturated fluorescent dye Eva Green that can simultaneously detect pigeon TTV and pigeon new adenovirus dual real-time fluorescent quantitative PCR detection method at home and abroad. The establishment of the present invention can fill the research gap in related fields at home and abroad

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