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Cosmetics containing spider silk hydrolyzate and preparation method thereof

A technology of spider silk and cosmetics, which is applied in the field of cosmetics containing spider silk hydrolyzate and its preparation, and achieves the effects of simple operation, high operation safety factor and low investment

Active Publication Date: 2020-09-15
HAINAN ZHUWANG PHARMA +1
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

Products using spider silk and its hydrolyzate as cosmetic raw materials have not been reported in the literature

Method used

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  • Cosmetics containing spider silk hydrolyzate and preparation method thereof
  • Cosmetics containing spider silk hydrolyzate and preparation method thereof
  • Cosmetics containing spider silk hydrolyzate and preparation method thereof

Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0050] Example 1 Preparation of spider silk hydrolyzate

[0051] The hydrolysis process of the present embodiment spider silk hydrolyzate is:

[0052] (1) Pretreatment: Take 2g of spider silk, rinse it with purified water to remove impurities, and put it in an agate ball mill for crushing until the spider silk has a flocculent or powdery growth length of <1 mm.

[0053] (2) Hydrolysis: Put the pulverized spider silk into a 1L glass container, add 200mL of 20g / L sodium hydroxide solution, and hydrolyze for 5h at a temperature of 80°C.

[0054] (3) Centrifugation: Centrifuge the hydrolyzate at a rotational speed of 15000r / min for 20min.

[0055] (4) Collection: Collect the centrifuged hydrolyzate into a glass container.

[0056] (5) Hydrolysis: hydrolyze the unhydrolyzed spider silk again, the method is the same as (2).

[0057] (6) Neutralization: neutralize with 1mol / L hydrochloric acid solution to pH 4.5.

[0058] (7) Filtration: filter the neutralized hydrolyzate through...

Embodiment 2

[0060] Example 2 Preparation of spider silk hydrolyzate

[0061] The hydrolysis process of the present embodiment spider silk hydrolyzate is:

[0062] (1) Pretreatment: Take 2g of spider silk, rinse it with purified water to remove impurities, and put it in an agate ball mill for crushing until the spider silk has a flocculent or powdery growth length of <1 mm.

[0063] (2) Hydrolysis: Put the pulverized spider silk into a 1L glass container, add 200mL of 15g / L sodium hydroxide solution, and hydrolyze for 4.5h at a temperature of 85°C.

[0064] (3) Centrifugation: Centrifuge the hydrolyzate at a rotational speed of 15000r / min for 20min.

[0065] (4) Collection: Collect the centrifuged hydrolyzate into a glass container.

[0066] (5) Hydrolysis: hydrolyze the unhydrolyzed spider silk again, the method is the same as (2).

[0067] (6) Neutralization: neutralize with 1mol / L hydrochloric acid solution to pH 5.0.

[0068] (7) Filtration: filter the neutralized hydrolyzate throu...

Embodiment 3

[0070] Example 3 Preparation of spider silk hydrolyzate

[0071] The hydrolysis process of the present embodiment spider silk hydrolyzate is:

[0072] (1) Pretreatment: Take 2g of spider silk, rinse it with purified water to remove impurities, and put it in an agate ball mill for crushing until the spider silk has a flocculent or powdery growth length of <1 mm.

[0073] (2) Hydrolysis: Put the pulverized spider silk into a 1L glass container, add 200mL of 25g / L sodium hydroxide solution, and hydrolyze for 4 hours at a temperature of 90°C.

[0074] (3) Centrifugation: Centrifuge the hydrolyzate at a rotational speed of 15000r / min for 20min.

[0075] (4) Collection: Collect the centrifuged hydrolyzate into a glass container.

[0076] (5) Hydrolysis: hydrolyze the unhydrolyzed spider silk again, the method is the same as (2).

[0077] (6) Neutralization: neutralize with 1mol / L hydrochloric acid solution to pH 5.5.

[0078] (7) Filtration: filter the neutralized hydrolyzate th...

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Abstract

The invention relates to the technical field of cosmetics, and especially relates to a cosmetic containing spider silk hydrolysate. The cosmetic comprises the spider silk hydrolysate, and the spider silk hydrolysate is prepared by the following steps: spider silk is crushed, crushed spider silk and a sodium hydroxide solution are mixed, hydrolysis is carried out at 80-90 DEG C for 4-5 hours, centrifugation is carried out, and hydrolysate is obtained; pH value of the hydrolysate is adjusted to 4.0-5.5, the hydrolysate is filtered with a 200nm membrane, and the spider silk hydrolysate is obtained. The hydrolysis yield of amino acids is higher than 70%, and the amount of micro-molecule polypeptides is 72% or above; in the hydrolysate, methionine is not detected, except phenylalanine and tyrosine, yield of other amino acids is higher than 60%. The spider silk hydrolysate is used for masks for beauty treatment and skin care, beauty treatment characteristics of spider silk for water supplement, moisture retention, tightness, crease resistance, inflammation resistance, repair and the like are fully performed, the product characteristics are distinct, efficacy is substantial, and beauty treatment demands of people are satisfied.

Description

technical field [0001] The invention relates to the technical field of cosmetics, in particular to a cosmetic containing spider silk hydrolyzate and a preparation method thereof. Background technique [0002] Environmental pollution, aging, climate change, lack of sleep, personal physique, fatigue, electromagnetic radiation and other reasons are all important causes of dry, sagging, damaged, allergic, and fragile skin. The main ways to keep the skin healthy and moisturized are: continuously provide skin moisture and nutrients, improve the allergic mechanism, repair damaged skin, and comprehensively improve the sub-healthy state of the skin. Therefore, cosmetics with moisturizing, firming and anti-wrinkle, anti-inflammatory and repairing effects are favored by beauty lovers. [0003] Fibroin is a fibrous protein of silk or spider silk. It is rich in amino acids that make up fibroin. It is easily absorbed by the skin and nourishes the skin. Niacinamide improves skin quality. ...

Claims

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Application Information

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Patent Type & Authority Patents(China)
IPC IPC(8): A61K8/64A61K8/98A61K8/73A61K8/67A61Q19/00
Inventor 朱一强李平曾青钦
Owner HAINAN ZHUWANG PHARMA
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