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Anti-aging medicine based on dihydromyricetin

A technology for dihydromyricetin and anti-aging, which can be applied in the directions of drug combination, antidote, and pharmaceutical formulation, and can solve the problem of high preparation cost

Inactive Publication Date: 2017-12-22
SICHUAN AGRI UNIV
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

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Problems solved by technology

[0003] In summary, the existing problems in the prior art are: the preparation cost of existing anti-aging drugs is high, and long-term use brings health risks such as tumors

Method used

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  • Anti-aging medicine based on dihydromyricetin
  • Anti-aging medicine based on dihydromyricetin

Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0013] Embodiment 1, the life-prolonging effect of dihydromyricetin

[0014] Dihydromyricetin was purchased from sigma (product number: SML0295), and was dissolved in ethanol as a stock solution of 100mM / L for later use. The experimental animal is the model animal Drosophila, the strain is: Dahomey, the temperature: 25°C, the relative humidity: 65% culture; the drug is added to the food according to the final concentration: 10um / L, 40um / L, 100um / L to feed the experimental Drosophila. Count the number of deaths, draw survival curves, and analyze the ability of drugs to prolong aging, such as figure 1 shown.

[0015] Table 1, Drosophila lifespan statistical table (where the red mark is p<0.05, the treatment with significant statistical difference)

[0016]

[0017] The results showed that 40um / L dihydromyricetin can significantly anti-aging and prolong the lifespan of Drosophila.

Embodiment 2

[0018] Example 2, dihydromyricetin can increase the number of intracellular bodies in intestinal cells.

[0019] After being fed with dihydromyricetin for 30 days, the intestines of Drosophila in the control group and the dihydromyricetin-treated group were dissected, rinsed twice with 10% PBS, and stained with 1 μM LysoTracker DS Red DND-99 (Invitrogen, Molecular Probes) for 3 minutes , and then quickly rinsed with PBS for one time, sealed the slides, observed and photographed autophagosomes under a fluorescence microscope at 545 nm, and processed the images with imageJ to count the number of autophagosomes. The result is as figure 2 . It can be seen that dihydromyricetin treatment can significantly increase the number of autophagosomes in intestinal cells, indicating that dihydromyricetin can increase the level of autophagy in cells.

[0020] The dihydromyricetin described in the invention can be used as an active ingredient and can be applied to anti-aging medicines, thu...

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Abstract

The invention belongs to the technical field of anti-aging medicines, and discloses an anti-aging medicine based on dihydromyricetin. The anti-aging medicine based on dihydromyricetin is 50 Mm / L of dihydromyricetin ethanol solution. The dihydromyricetin in the invention has obvious effects of resisting aging and prolonging the life-span of drosophila; compared with a control group, the dihydromyricetin can prolong the half life-span of drosophila by 14.29% and the maximum life-span of drosophila by 10.58%; the mechanism research indicates that the dihydromyricetin can reduce a horizontal activated AMPK signal channel of ATP inside cells, so that the autophagy related gene expression level is improved, the quantity of autophagic vacuoles inside the cells is increased, the senescence is delayed, and the life span is prolonged. Experimental results indicate that the dihydromyricetin has an anti-aging effect.

Description

technical field [0001] The invention belongs to the technical field of anti-aging drugs, in particular to an anti-aging drug based on dihydromyricetin. Background technique [0002] Dihydromyricetin (DHM) is a dihydroflavonol flavonoid compound, which was first isolated from the leaves of Ampelopsis meliaefolia (Hand.-Mazz.) W.T.Wang in 1940. The amount in vine tea can reach 30%. The chemical name of dihydromyricetin is (2R,3R)-3,5,7-trihydroxy-2-(3,4,5-trihydroxyphenyl)benzodi Hydropyran-4-one, the molecular formula is C15H12O8, and the relative molecular mass is 320.25. Vine tea has a long history of being used as a health tea in Fujian, Hunan, and Hubei. Previous studies have confirmed that dihydromyricetin has various pharmacological effects such as anti-oxidation, anti-tumor, anti-inflammation, anti-alcoholic and liver protection, anti-pathogenic microorganisms and blood lipid regulation. Studies in recent years have shown that dihydromyricetin has a wide range of ph...

Claims

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Application Information

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IPC IPC(8): A61K31/352A61P39/06
CPCA61K31/352
Inventor 樊晓兰杨明耀崔浪曾姚杨德英毛雪平
Owner SICHUAN AGRI UNIV
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