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Gene and protein participating in stress response of tobacco salt, and application of gene and protein

A salt stress and gene technology, applied in the field of molecular biology, can solve problems such as unknown functions, and achieve the effect of good salt resistance and ability to improve

Inactive Publication Date: 2017-12-08
SICHUAN AGRI UNIV
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

Thirteen CBL genes have been cloned from broccoli, among which CBL10 is involved in the response to salt stress. At present, the functions of different CBL genes in common tobacco are unknown

Method used

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  • Gene and protein participating in stress response of tobacco salt, and application of gene and protein
  • Gene and protein participating in stress response of tobacco salt, and application of gene and protein
  • Gene and protein participating in stress response of tobacco salt, and application of gene and protein

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Experimental program
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Embodiment 1

[0032] 1. Gene cloning

[0033] Take 0.5 g of fresh tobacco leaves, use Trizol method to extract total RNA of tobacco cells, then use TaKaRa company's cDNA synthesis kit to synthesize cDNA, and then use Primer5.0 software to design primers for PCR amplification of cDNA. Amplified target gene detection results such as figure 1 , The length of the fragment is about 700bp. After the target fragment is purified, it is ligated with the pMD19-T vector overnight at 16°C. The ligated product is transformed into E. coli DH5α competent, and then screened on an LB plate coated with ampicillin, and positive clones are detected by colony PCR. After the detection, 3 independent positive clones were randomly selected and sent to a biotechnology company for sequencing. The gene sequence is shown in SEQ ID NO.1.

[0034] 2. The target gene is connected to the expression vector

[0035] The above-mentioned transformed T-vector and expression vector PBI121 were subjected to double enzyme diges...

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Abstract

The invention relates to a gene and a protein participating in stress response of tobacco salt, and application of the gene and the protein, and belongs to the technical field of molecular biology. The invention provides the gene CBL3 participating in stress response of the tobacco salt, and a nucleotide sequence of the gene is shown in SEQ ID NO.1. Through excessive expression of the gene CBL3 provided by the invention in tobacco, the ability of resisting salt stress by the tobacco is greatly promoted, and the gene CBL3 provided by the invention has a good anti-salt effect.

Description

technical field [0001] The invention relates to the technical field of molecular biology, in particular to a gene and protein involved in tobacco salt stress response and application thereof. Background technique [0002] Expression of plant-like calcineurin B-like protein (CBL) family genes has important functions in the process of stress response, and has been cloned from many plants (Arabidopsis thaliana, melon, tobacco, etc.) To this family gene (Xu Jiang et al. 2006; Liu Mingyu et al. 2015; Dong Honglian, 2015). CBL1 loss-of-function mutants in Arabidopsis are sensitive to aluminum (Osena, 2017), and tobacco CBL10 can respond to physiological processes of high salt, low potassium, high temperature and drought. Thirteen CBL genes were cloned from broccoli, among which CBL10 was involved in the response to salt stress. At present, the functions of different CBL genes in common tobacco are unknown. Contents of the invention [0003] The purpose of the present invention...

Claims

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Application Information

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Patent Type & Authority Applications(China)
IPC IPC(8): C12N15/55C12N9/16C12N15/82A01H5/00
CPCC12N9/16C12N15/8273C12Y301/03016
Inventor 李立芹鲁黎明张雪微卓维陈倩杨尚瑜鲁逸飞
Owner SICHUAN AGRI UNIV
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