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A gene, protein and application thereof involved in tobacco salt stress response

A salt stress and gene technology, applied in the field of molecular biology, can solve problems such as unknown functions, and achieve the effect of good salt resistance and ability to improve

Inactive Publication Date: 2019-11-26
SICHUAN AGRI UNIV
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

Thirteen CBL genes have been cloned from broccoli, among which CBL10 is involved in the response to salt stress. At present, the functions of different CBL genes in common tobacco are unknown

Method used

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  • A gene, protein and application thereof involved in tobacco salt stress response
  • A gene, protein and application thereof involved in tobacco salt stress response
  • A gene, protein and application thereof involved in tobacco salt stress response

Examples

Experimental program
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Effect test

Embodiment 1

[0032] 1. Gene cloning

[0033] Take 0.5g of fresh tobacco leaves, extract the total RNA of tobacco cells by the Trizol method, and then synthesize cDNA with TaKaRa's cDNA synthesis kit, and further use Primer5.0 software to design primers for PCR amplification of cDNA. Amplification target gene test results such as figure 1 The length of the fragment is about 700bp. After purification, the target fragment is ligated with pMD19-T vector at 16°C overnight. The ligation product is transformed into E. coli DH5α competent, and then screened on LB plates coated with ampicillin. Colony PCR is used to detect positive clones. After the detection, 3 independent positive clones were randomly selected and sent to the biotechnology company for sequencing. The gene sequence is shown in SEQ ID NO.1.

[0034] 2. Target gene connection expression vector

[0035] The above-mentioned transformed T-vector and expression vector PBI121 were respectively subjected to double enzyme digestion (restriction ...

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Abstract

The invention relates to a gene and a protein participating in stress response of tobacco salt, and application of the gene and the protein, and belongs to the technical field of molecular biology. The invention provides the gene CBL3 participating in stress response of the tobacco salt, and a nucleotide sequence of the gene is shown in SEQ ID NO.1. Through excessive expression of the gene CBL3 provided by the invention in tobacco, the ability of resisting salt stress by the tobacco is greatly promoted, and the gene CBL3 provided by the invention has a good anti-salt effect.

Description

Technical field [0001] The invention relates to the technical field of molecular biology, in particular to a gene and protein involved in the salt stress response of tobacco and their applications. Background technique [0002] Expression of plant calcineurin B-like protein (CBL) family genes has important functions in the process of stress response, and has been cloned from many plants (Arabidopsis thaliana, melon, forest tobacco, etc.) To this family gene (Xu Jiang et al. 2006; Liu Mingyu et al. 2015; Dong Honglian, 2015). The CBL1 mutant in Arabidopsis thaliana is sensitive to aluminum (Osena, 2017). Tobacco CBL10 can respond to the physiological processes of high salt, low potassium, high temperature and drought. Thirteen CBL genes were cloned from broccoli, of which CBL10 is involved in salt stress response. At present, the functions of different CBL genes in common tobacco are unknown. Summary of the invention [0003] The purpose of the present invention is to provide a g...

Claims

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Application Information

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Patent Type & Authority Patents(China)
IPC IPC(8): C12N15/55C12N9/16C12N15/82A01H5/00A01H6/82
CPCC12N9/16C12N15/8273C12Y301/03016
Inventor 李立芹鲁黎明张雪微卓维陈倩杨尚瑜鲁逸飞
Owner SICHUAN AGRI UNIV
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