Method for measuring content of free hemoglobin by multi-position transmission spectrum and fluorescent spectrum

A technology of fluorescence spectroscopy and transmission spectroscopy, which is applied in the field of chemical measurement of complex solution concentration analysis, can solve the problems of unsatisfactory chemical inspection methods, unresponsive response, poor pertinence, etc., achieve pollution-free high-precision measurement, and suppress nonlinearity problem, suppressing the effects of nonlinear

Inactive Publication Date: 2017-11-24
TIANJIN UNIV
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

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Problems solved by technology

[0002] Among the existing technologies, the more mature technology is to detect the content of free hemoglobin in blood bags through chemical testing, which has the outstanding advantage of high accuracy, but the method of chemical testing cannot meet the needs of fast, non-contact, and pollution-free. Due to its non-contact and pollution-free characteristics, spectroscopic measurement may also detect the content of free hemoglobin in blood bags
[0003] In view of the complexity of blood components, the pure transmission spectrum obtains the information of whole blood, which is poor in pertinence. In order to further improve the measurement accuracy of free hemoglobin, combined with the strong pertinence of fluorescence, but affected by the strong scattering of blood, Therefore, it cannot reflect the characteristics of the measured substance very well.

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  • Method for measuring content of free hemoglobin by multi-position transmission spectrum and fluorescent spectrum
  • Method for measuring content of free hemoglobin by multi-position transmission spectrum and fluorescent spectrum
  • Method for measuring content of free hemoglobin by multi-position transmission spectrum and fluorescent spectrum

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Embodiment 1

[0050] In the method for measuring the content of free hemoglobin by multi-position transmission and fluorescence spectroscopy provided by the embodiments of the present invention, the devices used are such as figure 1 As shown, it includes: a light source 1 , a blood bag 3 , a displacement platform 4 and a spectrum receiving device 5 .

[0051] Wherein, ensure that the light outlet of the light source 1 and the incident slit of the spectrum receiving device 5 are close to the blood bag 3, the light source 1 transmits and excites the blood sample in the blood bag 3 at the first position a, and the transmission spectrum is collected by the spectrum receiving device 5 Spectrum and fluorescence spectrum; control the light source to move to the second position b through the displacement platform 4, and collect the transmission spectrum and fluorescence spectrum at this position; control the light source to move to position n through the displacement platform 4, and collect the tran...

Embodiment 2

[0060] The difference between the embodiment of the present invention and embodiment 1 is only that the light source 3 and the movement mode of the spectrum receiving device 7 are different, see the following description for details:

[0061] see figure 2 Ensure that the light outlet of the light source 1 and the incident slit of the spectrum receiving device 5 are close to the blood bag 3, the light source 1 transmits and excites the blood sample in the blood bag 3, and the spectrum receiving device 5 collects the transmission spectrum and the fluorescence spectrum. Control the spectrum receiving device 7 to move to the second position b through the displacement platform 6, collect the transmission spectrum and fluorescence spectrum at this position, then control the spectrum receiving device 7 to move to position n through the displacement platform 6, and collect the transmission spectrum at this position and fluorescence spectra.

[0062] Wherein, the subsequent steps of ...

Embodiment 3

[0066] In actual implementation, due to the limitation of the space structure, it may occur that the light source 1 and the spectrum receiving device 5 cannot be close to the blood bag 3. At this time, an optical fiber can be respectively arranged at the light source 1 and the spectrum receiving device 5 as the incident optical fiber 2 and outgoing fiber 6.

[0067] see image 3 , the light source 1 transmits and excites the blood sample in the blood bag 3 through the incident optical fiber 2, and the spectrum receiving device 5 collects the transmission spectrum and the fluorescence spectrum through the outgoing optical fiber 6, and the incident optical fiber 2 and the outgoing optical fiber 6 are respectively close to the blood bag 3, Position a is the first position of the incident optical fiber 2, and the spectrum receiving device 5 collects the transmission spectrum and the fluorescence spectrum at this position; the incident optical fiber 2 is controlled by the displacem...

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Abstract

The invention discloses a method for measuring content of free hemoglobin by multi-position transmission spectrum and fluorescent spectrum. The method comprises the following steps of enabling a transmission light source to transmit a blood sample, enabling a fluorescent excitation light source to excite the blood sample, enabling a displacement platform to control the transmission light source and the fluorescent excitation light source to move to a plurality of positions to respectively transmit and excite the blood sample, and enabling a spectrum receiving device to collect transmission spectrum and fluorescent spectrum; normalizing the transmission spectrum and fluorescent spectrum at the plurality of positions, combining with chemical inspection data, and establishing a mathematical model; collecting the transmission spectrum and fluorescent spectrum at the plurality of positions of the blood sample, normalizing, and substituting into the mathematical model to calculate, so as to obtain the content of free hemoglobin.

Description

technical field [0001] The invention relates to the field of concentration analysis stoichiometry of complex spectral solutions, in particular to a method for measuring free hemoglobin content by combining multi-position transmission and fluorescence spectroscopy. Background technique [0002] Among the existing technologies, the more mature technology is to detect the content of free hemoglobin in blood bags through chemical testing, which has the outstanding advantage of high accuracy, but the method of chemical testing cannot meet the needs of fast, non-contact, and pollution-free. Due to its non-contact and pollution-free characteristics, spectroscopic measurement may also detect the content of free hemoglobin in blood bags. [0003] In view of the complexity of blood components, the pure transmission spectrum obtains the information of whole blood, which is poor in pertinence. In order to further improve the measurement accuracy of free hemoglobin, combined with the str...

Claims

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Application Information

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Patent Type & Authority Applications(China)
IPC IPC(8): G01N21/64
Inventor 林凌罗永顺张盛昭甄建芸王玉宇王艳军李刚
Owner TIANJIN UNIV
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