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A method of rapid expansion of cannabis

A marijuana, rapid technology, applied in the field of plant propagation, can solve the problems of reduced germination rate, limited quantity, unsatisfactory needs, etc., and achieve the effect of simple cultivation process, high genetic consistency, and short propagation cycle

Active Publication Date: 2020-05-26
INST OF BAST FIBER CROPS CHINESE ACADEMY OF AGRI SCI
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

The number and speed of its reproductive plants are limited, which cannot meet the needs
[0005] From the perspective of the preservation and utilization of seed resources, there are currently nearly a thousand hemp resources in the national germplasm resource bank, but because the hemp seeds are not resistant to storage, the germination rate is decreasing year by year, and some rare resources not only have low germination rates, but also extremely large quantities limited, which limits its application

Method used

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  • A method of rapid expansion of cannabis

Examples

Experimental program
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Effect test

Embodiment 1

[0030] The method for rapidly multiplying the marijuana of the present embodiment comprises the following implementation steps:

[0031] (1) Disinfection of seeds: select hemp seeds, perform disinfection and sterilization treatment, and inoculate on MS medium; hemp seeds germinate and grow into sterile seedlings;

[0032] Disinfection and sterilization treatment is an existing technology, and the following treatment methods are adopted:

[0033] ① After soaking in industrial sulfuric acid with a mass concentration of 98% for 20 minutes, rinse it under tap water for 30 minutes;

[0034] ② Soak with alcohol with a volume concentration of 75% for 2 minutes;

[0035] ③ Soak in 3% sodium hypochlorite solution for 20 minutes, rinse with sterile water, dry the water on clean filter paper, and peel off the seed coat with a scalpel and tweezers.

[0036] (2) Hydroponic cultivation: After the hemp seeds were inoculated on MS medium and cultivated for 4 days, the aseptic seedlings grew...

Embodiment 2

[0044] The method for rapidly multiplying the marijuana of the present embodiment comprises the following implementation steps:

[0045] (1) Disinfection of seeds: select hemp seeds, perform disinfection and sterilization treatment, and inoculate on MS medium; hemp seeds germinate and grow into sterile seedlings;

[0046] Disinfection and sterilization treatment is an existing technology, and the following treatment methods are adopted:

[0047] ① After soaking in industrial sulfuric acid with a mass concentration of 98% for 20 minutes, rinse it under tap water for 30 minutes;

[0048] ② Soak with alcohol with a volume concentration of 75% for 2 minutes;

[0049] ③ Soak in 3% sodium hypochlorite solution for 20 minutes, rinse with sterile water, dry the water on clean filter paper, and peel off the seed coat with a scalpel and tweezers.

[0050](2) Hydroponic cultivation: After the hemp seeds were inoculated on MS medium and cultivated for 7 days, the sterile seedlings grew ...

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Abstract

The invention relates to a rapid hemp propagation method. The rapid hemp propagation method comprises the following implementation steps: (1) disinfecting seeds; (2) carrying out water culture; (3) transferring hemp seedlings into a culture medium; (4) topping the hemp seedlings; (5) carrying out rooting culture on lateral buds; (6) carrying out enrichment culture; and (7) carrying out seedling hardening. The rapid hemp propagation method has the advantages of short propagation cycle, high multiplication coefficient, simple culture process, high genetic uniformity and the like. The method is utilized for carrying out tissue culture propagation on hemp seeds, 8 auxiliary buds are extracted from each substance seedling through calculation by taking 100 days as a period, and 500 seedlings can be propagated by virtue of each seed seedling, so that the rapid propagation problem of excellent or valuable and rare hemp varieties in a short cycle can be effectively solved, hemps can be stored or propagated under the condition that the genetic uniformity is guaranteed, and meanwhile, a foundation is laid for industrialized seedling production. The method is particularly applicable to valuable and rare seeds with low germination rate and number.

Description

technical field [0001] The invention relates to the technical field of plant propagation, in particular to a method for efficiently and rapidly multiplying cannabis. [0002] technical background [0003] Cannabis (Cannabis Sativa L.) is an annual herbaceous plant belonging to the family Cannabinaceaceae and has a history of cultivation and utilization for thousands of years in my country. Hemp is full of treasures. It can not only produce high-quality textile fibers, but also has great application value in food, medicine and building materials industries. According to its tetrahydrocannabinol (THC) content, it is divided into three types: drug marijuana, intermediate marijuana and fiber marijuana. my country is a big country in the cultivation of fiber marijuana, and the planting area accounts for about 30% of the world. In recent years, medicinal marijuana has also become the focus of attention in the industry. Its medicinal ingredient CBD (cannabidiol) is a non-addictive ...

Claims

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Application Information

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Patent Type & Authority Patents(China)
IPC IPC(8): A01G31/00C05G1/00
CPCA01G31/00C05B7/00C05D9/00C05G5/23C05F11/02C05C5/02C05C5/04C05D5/00C05D9/02
Inventor 程超华粟建光唐蜻戴志刚方冬兵文海兵许英杨泽茂高红刘婵
Owner INST OF BAST FIBER CROPS CHINESE ACADEMY OF AGRI SCI
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