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Application of juglone in preparation of TRAIL sensitizer

A technology of juglone and sensitizer, which is applied in the field of biomedicine and can solve problems such as TRAIL sensitizers that have not yet been seen

Inactive Publication Date: 2017-10-31
SHANDONG PROVINCIAL HOSPITAL
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

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Problems solved by technology

So far, there has been no report about juglone as a TRAIL sensitizer

Method used

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  • Application of juglone in preparation of TRAIL sensitizer
  • Application of juglone in preparation of TRAIL sensitizer
  • Application of juglone in preparation of TRAIL sensitizer

Examples

Experimental program
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Embodiment 1

[0018] Materials and Methods:

[0019] 1. Cell lines: Human melanoma cell lines (MEWO, A2058) were purchased from ATCC. The above cell lines were incubated in DMEM medium containing 10% fetal bovine serum at 37°C with a volume fraction of 5% CO 2 , Routine culture under fully saturated humidity conditions, replace the medium for 48 hours, when the cell growth reaches saturation, digest with 0.25% trypsin + 0.02% EDTA for passage, once every 2-3 days, the experiment uses logarithmic growth phase cells.

[0020] 2. Detection of cytotoxicity: adjust the concentration of MEWO and A2058 cell suspensions to 1×10 5 / mL, added to 96-well culture plate, 100 μL per well, placed at 37°C in 5% CO 2 After culturing in the incubator for 24 hours, the old medium was aspirated and cultured in a new DMEM medium containing 10% fetal bovine serum. Melanoma cells were pretreated with juglone (10, 20 μM) for 6 hours, washed once with PBS, replaced with fresh medium containing FBS, and TRAIL (25...

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Abstract

The invention belongs to the field of biomedicines, relates to a novel medicinal application of a natural drug active ingredient juglone, and particularly relates to an application of juglone in preparation of a TRAIL sensitizer. An experiment proves that the killing effect of a TRAIL on two tumor cell lines, namely human melanoma cell lines MEWO and A2058 can be significantly strengthened through combined utilization of the juglone and the TRAIL, and separate application of the RAIL does not have an obvious effect on cell proliferation and apoptosis of the human melanoma cell lines MEWO and A2058.

Description

technical field [0001] The invention belongs to the field of biomedicine, and relates to a new medicinal application of juglone, an active ingredient of natural medicine, in particular to the application of juglone in preparing a TRAIL sensitizer. Background technique [0002] Related literature discloses that tumor necrosis factor-related apoptosis-inducing ligand (TRAIL) is a new member of the tumor necrosis factor superfamily. Studies have shown that the TRAIL binds to death receptors and induces rapid and efficient apoptotic responses in tumor cells through various signal transduction pathways without obvious toxicity to normal cells. It is expected to become a new tumor treatment method. However, more and more studies have shown that some tumor cells have low sensitivity to TRAIL's apoptosis or even completely tolerate it. Therefore, it is very necessary to sensitize the anti-tumor effect of TRAIL. [0003] Juglone exists in the immature exocarp of Juglandaceae Juglans...

Claims

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Application Information

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Patent Type & Authority Applications(China)
IPC IPC(8): A61K31/122A61P35/00
CPCA61K31/122
Inventor 刘英超陈燕吴金峰葛明旭
Owner SHANDONG PROVINCIAL HOSPITAL
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