Application of juglone in preparation of TRAIL sensitizer
A technology of juglone and sensitizer, which is applied in the field of biomedicine and can solve problems such as TRAIL sensitizers that have not yet been seen
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[0018] Materials and Methods:
[0019] 1. Cell lines: Human melanoma cell lines (MEWO, A2058) were purchased from ATCC. The above cell lines were incubated in DMEM medium containing 10% fetal bovine serum at 37°C with a volume fraction of 5% CO 2 , Routine culture under fully saturated humidity conditions, replace the medium for 48 hours, when the cell growth reaches saturation, digest with 0.25% trypsin + 0.02% EDTA for passage, once every 2-3 days, the experiment uses logarithmic growth phase cells.
[0020] 2. Detection of cytotoxicity: adjust the concentration of MEWO and A2058 cell suspensions to 1×10 5 / mL, added to 96-well culture plate, 100 μL per well, placed at 37°C in 5% CO 2 After culturing in the incubator for 24 hours, the old medium was aspirated and cultured in a new DMEM medium containing 10% fetal bovine serum. Melanoma cells were pretreated with juglone (10, 20 μM) for 6 hours, washed once with PBS, replaced with fresh medium containing FBS, and TRAIL (25...
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