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Immunochromatographic test strip for determining IgG anti-A and anti-B blood group antibodies of pregnant woman or human blood-group reverse typing

An immunochromatographic test strip, blood group antibody technology, applied in biological tests, measuring devices, analytical materials, etc., can solve the problems of time-consuming and energy-consuming, complex measurement process, errors or inaccuracy, etc. Cumbersome, intuitive results interpretation

Active Publication Date: 2017-10-24
邵超鹏
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

Due to the complexity of the determination process, most hospitals currently use the micro-column gel card method, which can omit the steps of red blood cell washing and microscope interpretation, but the whole process is still time-consuming, cumbersome and expensive
[0008] The applicant has been engaged in blood type research for more than 20 years, and always believes that the determination of IgG anti-A and anti-B antibody titers in prenatal serum of pregnant women in clinical laboratories consumes too much time and energy for routine staff, and errors or inaccuracies often occur Occurs, so the traditional determination methods urgently need to be improved or replaced

Method used

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  • Immunochromatographic test strip for determining IgG anti-A and anti-B blood group antibodies of pregnant woman or human blood-group reverse typing

Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0045] Example 1 Determining the IgG anti-A and anti-B blood group antibodies of pregnant women or the immunochromatographic test strips of human blood group reverse typing

[0046] It includes a sample pad 1, a tracer pad 2, a cellulose membrane 3, a blocking area 4, a detection area 5, a quality control area 6, an absorbent pad 7 and a support plate 8; the support plate 8 is located at the bottom, and the support plate 8 is glued There is a cellulose film 3, and the two ends of the cellulose film 3 are respectively lapped with a tracer pad 2 and a water-absorbent pad 7; the other end of the tracer pad 2 is lapped with a sample pad 1;

[0047] The sample pad 1 is dripped with the sample to be tested, and the tracer pad 2 is coated with a tracer; the cellulose membrane 3 is sequentially provided with a blocking area 4, a detection area 5 and a quality control area 6;

[0048] The blocking area 4 is coated with human IgM-specific binders; the detection area 5 is coated with blo...

Embodiment 2

[0055] Embodiment 2 The immunochromatographic test strip of human blood group reverse typing

[0056] The structure is the same as that in Example 1. When used to determine the reverse typing of human blood type, the coating material on the blocking area 4 is a human IgG specific binding substance; the detection area 5 is coated with blood type A antigen conjugate, B antigen conjugate One or more of reactants of anti-human IgM, human IgM-specific binders or tracers;

[0057] When the blocking region is coated with one or more of anti-human IgG antibody or protein A or protein G or human IgG specific binders, the specific reactant of human IgM antibody is used to label the tracer particle at the same time. It can specifically detect IgM anti-A and anti-B antibodies;

[0058] The IgG detection area 5 and the quality control area 6 can be set at the same time. When there are one or more detection lines on the same membrane, the quality control area can be set up or not;

[0059...

Embodiment 3

[0060] Example 3 An immunochromatographic test strip method (protein A gold standard method) for measuring IgG anti-A and anti-B antibody titers in pregnant women's serum or plasma, the main steps are as follows:

[0061] 1. Sample pad 1 is a serum sample pad;

[0062] 2. Production of the tracer and tracer pad 2: the tracer includes tracer particles and tracer particle conjugates, the tracer particles are selected from colloidal gold, and the tracer particle conjugate is protein A, after the colloidal gold is labeled with protein A It is the tracer, and then the tracer is dispersed and adsorbed on the glass fiber mat and freeze-dried to be the tracer pad 2;

[0063] 3. Preparation of Antigen Modifier A and Antigen B Modifier: Using solid-phase synthesis method, amino group is selected as the connecting arm, resin is selected as the carrier, and a fully protected glycosyl donor (the donor has a leaving group) is activated Under the action of a reagent, it is coupled with a li...

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Abstract

The invention relates to an immunochromatographic test strip for determining IgG anti-A and anti-B blood group antibodies of a pregnant woman or human blood-group reverse typing, and belongs to the technical field of biological medicines. The immunochromatographic test strip comprises a sample pad, a tracing pad, a cellulose membrane, an IgM blocking area, an IgG detecting area, a quality control area, a water absorbing pad and a supporting plate, wherein the supporting plate is positioned at the bottom part and is provided with the cellulose membrane, and the two ends of the cellulose membrane are respectively overlapped with the tracing pad and the water absorbing pad; and the sample pad is overlapped on the other end of the tracing pad. The immunochromatographic test strip has the advantages that the existence and the titer of human blood IgG-type anti-A and anti-B antibodies can be simply and rapidly determined in a specific manner, the traditional and current determination mode 'IgM inactivation, anti-human ball test and agglutination observation 'is replaced, the operation steps are greatly simplified, and the time consumed is shortened; and the immunochromatographic test strip also can be used for determining the reverse typing of human blood.

Description

technical field [0001] The invention relates to an immunochromatographic test strip for measuring IgG anti-A and anti-B blood group antibodies of pregnant women or human blood type antityping, which can be used for the determination of prenatal IgG anti-A and anti-B antibodies and potency of pregnant women , to predict the hemolytic disease of newborns; it can also be used to identify the reverse stereotyping of individual blood types, and belongs to the technical field of biomedicine. Background technique [0002] Many invention patents use immunochromatographic test strip technology to establish specific detection methods. Their technologies are basically the same. The difference is that either the detection objects are different or the detection purposes are different. [0003] Several invention patents or utility model patents, including rejected or unauthorized patent application materials, all describe the use of immunochromatographic test strip technology to determine...

Claims

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Application Information

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IPC IPC(8): G01N33/532G01N33/558G01N33/58G01N33/80
CPCG01N33/532G01N33/558G01N33/58G01N33/80
Inventor 邵超鹏
Owner 邵超鹏
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