Solid inoculant for making silage corn and preparing and using methods thereof
A technology of solid inoculum and silage corn, which is applied in microorganism-based methods, biochemical equipment and methods, preservation methods of animal feed raw materials, etc. Corn, the effect of increasing lactic acid content
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[0068] The present invention provides the preparation method of above-mentioned solid bacterial agent, comprises the steps:
[0069] Mix beneficial bacteria strain A with molasses, and carry out aerobic fermentation at 15-40°C for 18-30 hours to obtain fermentation product A;
[0070] Mix beneficial bacteria strain B with cassava dry residue and palm kernel meal powder, and aerobically ferment at 15-40°C for 18-30 hours to obtain fermentation product B;
[0071] The fermentation product A and the fermentation product B are mixed, and anaerobically fermented for 12-18 days at 15-40° C. under the condition of avoiding light, to obtain a solid bacterial agent for making silage corn.
[0072] The purposes, effects and preferred solutions of the above steps have been clearly described above, and will not be repeated here.
[0073] The present invention provides a method for using the solid bacterial agent described in the above technical solution or the solid bacterial agent obtai...
Embodiment 1
[0080] (1) Preparation of strains:
[0081] Bacteria powder A: including 2×10 9 CFU / g active yeast, 1×10 9 CFU / g of amylolytic bacteria;
[0082] Bacteria Powder B: Including 1×10 9 CFU / g Lactobacillus plantarum, 2×10 9 CFU / g of Lactobacillus clothi, 2×10 9 CFU / g Urinary Enterococcus, 2×10 9 CFU / g of Bacillus, 5×10 11 CFU / g of lactic acid bacteria, 5×10 11 CFU / g of cellulolytic bacteria.
[0083] (2) Preparation of solid bacterial agent:
[0084] Mix 2.5kg of bacteria powder A, 2.5kg of fructooligosaccharide and 7.5kg of water, activate and multiply at 25°C for 24 hours to obtain beneficial bacteria strain A; mix beneficial bacteria strain A with 300kg of molasses, Under the condition of aerobic fermentation for 24 hours, the fermentation product A was obtained.
[0085] Mix 2.5kg of bacteria powder B, 2.5kg of fructooligosaccharides and 7.5kg of water, activate and multiply at 25°C for 24 hours to obtain beneficial bacteria strain B; mix beneficial bacteria strain B...
Embodiment 2
[0092] (1) Preparation of strains:
[0093] Bacteria powder A: including 1×10 9 CFU / g active yeast, 0.5×10 9 CFU / g of amylolytic bacteria;
[0094] Bacteria powder B: including 0.5×10 9 CFU / g Lactobacillus plantarum, 1×10 9 CFU / g of Lactobacillus clothi, 1×10 9 CFU / g Urinary Enterococcus, 1×10 9 CFU / g of Bacillus, 2×10 11 CFU / g of lactic acid bacteria, 2×10 11 CFU / g of cellulolytic bacteria.
[0095] (2) Preparation of solid bacterial agent:
[0096] Mix 2.5kg of bacteria powder A, 2.5kg of fructooligosaccharide and 7.5kg of water, activate and multiply at 25°C for 24 hours to obtain beneficial bacteria strain A; mix beneficial bacteria strain A with 300kg of molasses, Under the condition of aerobic fermentation for 24 hours, the fermentation product A was obtained.
[0097] Mix 2.5kg of bacteria powder B, 2.5kg of fructooligosaccharides and 7.5kg of water, activate and multiply at 25°C for 24 hours to obtain beneficial bacteria strain B; mix beneficial bacteria stra...
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