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Novel liquid compound microecological preparation for livestock and poultry and preparation method thereof

A composite micro-ecological and liquid technology, applied in microorganism-based methods, biochemical equipment and methods, microorganisms, etc., can solve the problems of lack of synergistic fermentation mixed strains in liquid fermentation, few types of fermentation accelerators, and bottle expansion, and achieve promotion The effect of synergistic fermentation rate, saving fermentation time, and good synergistic fermentation ability

Active Publication Date: 2017-10-17
FUJIAN BRADY PHARMA CO LTD
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

However, the current liquid probiotics products still have problems that need to be solved urgently: ①Strict sterilization is required before liquid fermentation, which is complicated and time-consuming; ②Part of the bottled liquid probiotics products are bulging, which shortens the shelf life of the products; ③ Liquid fermentation lacks excellent synergistic fermentation mixed strains; ④There are few types of fermentation accelerators in liquid fermentation, and there is even a lack of medicinal and food homologous fermentation accelerators

Method used

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  • Novel liquid compound microecological preparation for livestock and poultry and preparation method thereof
  • Novel liquid compound microecological preparation for livestock and poultry and preparation method thereof
  • Novel liquid compound microecological preparation for livestock and poultry and preparation method thereof

Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0039] Embodiment 1--Isolation and identification of Enterococcus faecalis BD-1

[0040] 1. Isolation, enrichment and purification of Enterococcus faecalis BD-1

[0041] Under aseptic operation conditions, farmers in Xia Village, Zherong County, Ningde City, Fujian Province (coordinates: 119 。 51′59.7″E, 27 。 13′34.0″N) Caecum mucosal segment of self-supporting healthy adult pigs, scrape the mucosal mucus with a sterilized glass slide and put it in the EP tube, dilute it with sterile normal saline for 10 2 Take 0.1mL and spread it on the MRS agar plate containing bromocresol purple indicator (0.01%, g / mL). The colonies that turned yellow were streaked and purified, and the strains that were positive for Gram staining under the microscope and without spores were selected to contain bromocresol purple (0.01%, g / mL), tea polyphenols (0.01%, g / mL) and garlic (0.01%, g / mL) on the MRS agar plate for re-screening, and finally select the colony with obvious yellow color, smooth and...

Embodiment 2

[0075] Embodiment 2--Isolation and identification of Clostridium butyricum RCM-2

[0076] 1. Isolation, enrichment and purification of Clostridium butyricum RCM-2

[0077] Under aseptic operation conditions, farmers in Xia Village, Zherong County, Ningde City, Fujian Province (coordinates: 119 。 52′04.1″E, 27 。 13′32.7″N) The cecal mucosal segment of a healthy adult rooster bred in a closed mountain forest, the mucosal mucus was scraped with a sterilized glass slide and placed in an EP tube, diluted with sterile normal saline for 10 2 Then heated to 60°C for 30min, took 0.1mL and spread it on the RCM agar plate containing bromocresol purple indicator (0.01%, g / mL), after coating, put the RCM agar plate in the anaerobic incubator , cultured at 37°C for 48 hours, selected the colonies that turned yellow in the medium, and purified them by streaking, and selected the rod-shaped strains that were positive for Gram staining and had spores in the microscopic examination, and conta...

Embodiment 3

[0107] Embodiment 3--Enterococcus faecalis BD-1 and Clostridium butyricum RCM-2 co-grow

[0108] (1) Activation of the strain

[0109] Activation of Enterococcus faecalis BD-1: Rinse the slant of Enterococcus faecalis BD-1 with sterile saline in a sterile environment, wash off the bacterial lawn on the surface of the medium, and then inoculate it into MRS liquid medium. 37 ℃ static culture for 48h;

[0110] Activation of Clostridium butyricum RCM-2: Rinse the slant of the test tube of Clostridium butyricum RCM-2 with sterile saline in a sterile environment, wash off the bacterial lawn on the surface of the medium, and then inoculate it into MRS liquid medium medium, 37°C static culture for 48h;

[0111](2) Then inoculate the activation solution of Enterococcus faecalis BD-1, the activation solution of Clostridium butyricum RCM-2, and the combination of the two at a volume ratio of 1:1 in the In the 200 mL anaerobic serum bottle of the MRS liquid medium, the gradient of the ...

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Abstract

The invention belongs to the technical field of production of microecological preparations, and particularly relates to a novel liquid compound microecological preparation for livestock and poultry and a preparation method thereof. The preparation method comprises the following steps: (1) activating two fermenting strains; (2) preparing fermenting seed solutions; (3) mixing and co-fermenting the two fermenting strains; (4) mixing liquid and solid; (5) bottling. By the preparation method provided by the invention, the liquid fermentation rate can be accelerated and preparation of high-concentration bacterial liquid is facilitated; the obtained microecological preparation can effectively prevent diseases caused by staphylococcus aureus, micrococcus luteus and escherichia coli, can supplement intestinal probiotics of livestock and poultry and can help to control a heat stress syndrome of livestock and poultry; moreover, by the preparation method provided by the invention, a novel liquid preparation can be constructed through in-vitro compounding of the microecological preparation and a Chinese herbal extract, diversified operation is facilitated, and the process is simple; finally, due to the adoption of the preparation method provided by the invention, strict sterilizing operation is not needed, the working difficulty is significantly reduced, energy waste is reduced, and the cost is reduced.

Description

technical field [0001] The invention belongs to the technical field of production and preparation of probiotics, and in particular relates to a novel liquid compound probiotics for livestock and poultry and a preparation method thereof. Background technique [0002] my country is a large agricultural country, and the development of animal husbandry occupies an important proportion. According to the country's supply-side reforms in recent years, the scale and development of the national animal husbandry will greatly drive the development of the industry. Therefore, overall, the industrialization, scale and standardization of my country's animal husbandry The development of the industry will be further promoted. The development potential of my country's livestock and poultry breeding industry during the "13th Five-Year Plan" period is huge, with huge space and great significance. However, there is a serious phenomenon of antibiotic abuse in my country's aquaculture industry. In...

Claims

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Application Information

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IPC IPC(8): C12N1/20A61K35/744A61K36/752A61P31/04A61P1/00A61P39/00C12R1/145C12R1/01A61K35/742A61K31/353A61K31/10A61K31/702
CPCA61K31/10A61K31/353A61K31/702A61K35/742A61K35/744A61K36/00A61K36/36A61K36/734A61K36/752C12N1/20A61K2236/51A61K2236/39A61K2236/333A61K2236/331A61K2300/00
Inventor 潘慧青张炎达杨荣平肖建设赵齐林贺桂
Owner FUJIAN BRADY PHARMA CO LTD
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