Anti-cancer composition extracted from lindley eupatorium herb, and preparation method and application thereof
A composition, wild horse technology, applied in the direction of drug combination, medical preparations containing active ingredients, anti-tumor drugs, etc., can solve the problems of high risk and high cost, and achieve strong biological activity, convenient use, and clear medicinal substances Effect
- Summary
- Abstract
- Description
- Claims
- Application Information
AI Technical Summary
Benefits of technology
Problems solved by technology
Method used
Image
Examples
Embodiment 1
[0038] The preparation method of Yemazhui three sesquiterpene lactone natural compositions comprises the following steps:
[0039] A. Extraction: Yemachai herbs are powdered and passed through a 40-mesh sieve for later use. Take 7kg of coarse powder of Yemazhui medicinal material, add appropriate amount of 95% ethanol to cold soak for 48 hours, then extract by percolation, and collect percolation liquid. The volume of the total solvent for extraction is equivalent to 18 times of the quality of the medicinal material (a total of 126 L of 95% ethanol is used).
[0040] B. Extraction: Concentrate the percolation liquid obtained in step A under reduced pressure until it has no alcohol smell, add water to suspend to 2L, extract with 2L of ethyl acetate and 2L of n-butanol, extract three times with each extraction solvent, and combine each time The extracts of Yemazhui ethyl acetate extract and n-butanol extract were obtained respectively. Through in vitro anti-breast cancer activi...
Embodiment 2
[0046] A. Extraction: Take 7kg of Yemachai medicinal material, add 10 times the amount (70L) of 95% ethanol, and reflux extraction. The extraction time is 2 hours each time, and the extracts are extracted twice, and the two extracts are combined. A total of 140L of 95% ethanol was used.
[0047] B. Extraction: Concentrate the extract obtained in step A under reduced pressure until it has no alcohol smell, add water to suspend to 2L, extract with 2L of ethyl acetate and 2L of n-butanol respectively, extract three times with each extraction solvent, and combine each time The extracts of Yemazhui ethyl acetate and n-butanol were obtained respectively. Through the in vitro anti-breast cancer activity tracking, the active ingredients of Yemazhui anti-breast cancer were mainly concentrated in the ethyl acetate extract, and the ethyl acetate extract The solvent was recovered under reduced pressure to obtain 234.8 g of the ethyl acetate extract of Yemachai.
[0048] C. Column chromat...
Embodiment 3
[0052] A. Extraction: Yemachai herbs are powdered and passed through a 40-mesh sieve for later use. Take 7kg of coarse powder of Yemazhui medicinal material, add an appropriate amount of 95% ethanol to cold soak for 48 hours, and then extract by percolation. The volume of the extraction solvent is equivalent to 18 times the mass of the medicinal material (a total of 126L of 95% ethanol is used), and the percolation solution is collected.
[0053] B. Freeze-drying: Concentrate the percolate obtained in step A under reduced pressure until it has no alcohol smell, and dry the thick extract by freeze-drying to obtain 279.4 g of freeze-dried powder.
[0054] C. Column chromatography: Take about 190 g of the freeze-dried powder of the above-mentioned Yemazhui alcohol extract, put it on a 100-200 mesh normal-phase silica gel column for separation, and then use a plurality of different solvent gradients for elution, and each elution solvent is composed of Composed of petroleum ether a...
PUM
Abstract
Description
Claims
Application Information
- R&D Engineer
- R&D Manager
- IP Professional
- Industry Leading Data Capabilities
- Powerful AI technology
- Patent DNA Extraction
Browse by: Latest US Patents, China's latest patents, Technical Efficacy Thesaurus, Application Domain, Technology Topic, Popular Technical Reports.
© 2024 PatSnap. All rights reserved.Legal|Privacy policy|Modern Slavery Act Transparency Statement|Sitemap|About US| Contact US: help@patsnap.com