Anti-cancer composition extracted from lindley eupatorium herb, and preparation method and application thereof

A composition, wild horse technology, applied in the direction of drug combination, medical preparations containing active ingredients, anti-tumor drugs, etc., can solve the problems of high risk and high cost, and achieve strong biological activity, convenient use, and clear medicinal substances Effect

Inactive Publication Date: 2017-10-13
ZHEJIANG CHINESE MEDICAL UNIVERSITY
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Benefits of technology

This patented technology describes an improved version of yimenaxel that contains multiple components called “sesquioterpenoid lactic acids” (SLAs). These compounds have powerful pharmacology properties like antimicrobial or cytotoxic activities against breast tumors. They also enhance cell growth by stimulating stem cells towards differentiating them into new tissue types such as bone marrow stromal blood vessels. Overall this innovative discovery makes these molecules more useful than other drugs currently available on market due their ability to treat various medical conditions effectively without causing side effects.

Problems solved by technology

Technologies described in Japanese Patent describes an improved way to isolate pure sorghum or yarnlella fractions containing these molecular species called XLBPOLYLEANESA®, which exhibits good pharmaceutical properties against hepatoma cell lines. These isolated mixtures contain only one type of component - yellow oleate perillylacerbane lactic acid), while others involve multiple ones at once. Additionally, they require expensive equipment and time-consumption processes due to tedious work procedures involved. Therefore, developing a simpler and efficient solution for producing highly pure Saphenkoid LiXILIO3 without contaminating any unwanted elements remains challenging.

Method used

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  • Anti-cancer composition extracted from lindley eupatorium herb, and preparation method and application thereof
  • Anti-cancer composition extracted from lindley eupatorium herb, and preparation method and application thereof
  • Anti-cancer composition extracted from lindley eupatorium herb, and preparation method and application thereof

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Experimental program
Comparison scheme
Effect test

Embodiment 1

[0038] The preparation method of Yemazhui three sesquiterpene lactone natural compositions comprises the following steps:

[0039] A. Extraction: Yemachai herbs are powdered and passed through a 40-mesh sieve for later use. Take 7kg of coarse powder of Yemazhui medicinal material, add appropriate amount of 95% ethanol to cold soak for 48 hours, then extract by percolation, and collect percolation liquid. The volume of the total solvent for extraction is equivalent to 18 times of the quality of the medicinal material (a total of 126 L of 95% ethanol is used).

[0040] B. Extraction: Concentrate the percolation liquid obtained in step A under reduced pressure until it has no alcohol smell, add water to suspend to 2L, extract with 2L of ethyl acetate and 2L of n-butanol, extract three times with each extraction solvent, and combine each time The extracts of Yemazhui ethyl acetate extract and n-butanol extract were obtained respectively. Through in vitro anti-breast cancer activi...

Embodiment 2

[0046] A. Extraction: Take 7kg of Yemachai medicinal material, add 10 times the amount (70L) of 95% ethanol, and reflux extraction. The extraction time is 2 hours each time, and the extracts are extracted twice, and the two extracts are combined. A total of 140L of 95% ethanol was used.

[0047] B. Extraction: Concentrate the extract obtained in step A under reduced pressure until it has no alcohol smell, add water to suspend to 2L, extract with 2L of ethyl acetate and 2L of n-butanol respectively, extract three times with each extraction solvent, and combine each time The extracts of Yemazhui ethyl acetate and n-butanol were obtained respectively. Through the in vitro anti-breast cancer activity tracking, the active ingredients of Yemazhui anti-breast cancer were mainly concentrated in the ethyl acetate extract, and the ethyl acetate extract The solvent was recovered under reduced pressure to obtain 234.8 g of the ethyl acetate extract of Yemachai.

[0048] C. Column chromat...

Embodiment 3

[0052] A. Extraction: Yemachai herbs are powdered and passed through a 40-mesh sieve for later use. Take 7kg of coarse powder of Yemazhui medicinal material, add an appropriate amount of 95% ethanol to cold soak for 48 hours, and then extract by percolation. The volume of the extraction solvent is equivalent to 18 times the mass of the medicinal material (a total of 126L of 95% ethanol is used), and the percolation solution is collected.

[0053] B. Freeze-drying: Concentrate the percolate obtained in step A under reduced pressure until it has no alcohol smell, and dry the thick extract by freeze-drying to obtain 279.4 g of freeze-dried powder.

[0054] C. Column chromatography: Take about 190 g of the freeze-dried powder of the above-mentioned Yemazhui alcohol extract, put it on a 100-200 mesh normal-phase silica gel column for separation, and then use a plurality of different solvent gradients for elution, and each elution solvent is composed of Composed of petroleum ether a...

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PUM

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Abstract

The invention discloses an anti-cancer composition extracted from lindley eupatorium herb, and a preparation method and application thereof. The anti-cancer composition is separated from the lindley eupatorium herb by taking Eupalinolide G, Eupalinolide I and Eupalinolide J as main active components and sequentially using methods of percolation, normal-phase silica-gel column chromatography, reversed-phase medium-pressure column chromatography and the like. By using an MTT method and tests for humanized tumors and xenograft tumors in nude mice, in-vitro and in-vivo anti-breast cancer activities of three sesquiterpene lactone natural composition from the lindley eupatorium herb can be researched respectively. The result shows that the composition can be used for treating the breast cancer. The three sesquiterpene lactone natural composition from the lindley eupatorium herb, prepared by using the method, is high in biological activity, easy to operate and low in production cost, has the advantages of stable and controllable quality and the like, and can be used for preparing a medicine for treating the breast cancer.

Description

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Claims

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Application Information

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Owner ZHEJIANG CHINESE MEDICAL UNIVERSITY
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