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Method for identifying clinic infected pig enterococcus by MALDI-TOFMS technology

A technology for technical identification and enterococci, applied in measuring devices, material analysis through electromagnetic means, instruments, etc., can solve problems such as economic losses in the pig industry, achieve strong acquired drug resistance, avoid the spread of strains, and avoid The effect of the emergence of more drug-resistant strains

Inactive Publication Date: 2017-09-29
HENAN UNIV OF ANIMAL HUSBANDRY & ECONOMY
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Problems solved by technology

[0002] Enterococcus is a Gram-positive facultative anaerobic bacterium, and it is one of the common bacteria that contaminate animal food. Recently, reports on the pathogenicity of Enterococcus to animals have been increasing, especially in pigs aged 20 to 70 days. An infectious disease characterized by subcutaneous and parenchymal organ enlargement and hemorrhage, which occurs frequently in free-range and small-scale pig herds, causing serious economic losses to the pig industry

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  • Method for identifying clinic infected pig enterococcus by MALDI-TOFMS technology
  • Method for identifying clinic infected pig enterococcus by MALDI-TOFMS technology
  • Method for identifying clinic infected pig enterococcus by MALDI-TOFMS technology

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[0029] Below in conjunction with the accompanying drawings and examples, the specific implementation of the present invention will be further described in detail, the following examples are used to illustrate the present invention, but not to limit the scope of the present invention.

[0030] In this example, 10 strains of Enterococcus established in the library were clinical strains confirmed by 16S rRNA, and 33 strains of Enterococcus verified after the establishment of the library were clinical wild isolates. The quality control strain ATCC29212 was Enterococcus faecalis, all of which were donated by Henan Agricultural University.

[0031] The formic acid, acetonitrile, trifluoroacetic acid and absolute ethanol used in the experiment are all chromatographically pure provided by Fisher Company. BTS standard, matrix α-cyano-4-hydroxycinnamic acid (HCCA), and MSP96-hole target were provided by Bruker, Germany.

[0032] Gram-positive bacteria identification card (GPI; producti...

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Abstract

The invention relates to the field of bacteria detection, and particularly discloses a method for identifying clinic infected pig enterococcus by an MALDI-TOFMS technology. The method has the technical scheme that the method for identifying the clinic infected pig enterococcus by the MALDI-TOFMS technology is characterized by comprising the following steps that 1, a formic acid extraction method is used for sample processing and sample application; 2, the sample treated in the first step is subjected to mass spectrometry by using an MALDI Biotyper high-flux microbiological identification system; a mass spectrum is obtained; 3, BioTyper software is used for analyzing the obtained mass spectrum to obtain the standard atlas of the enterococcus mass spectrum diagram; 4, the enterococcus to be detected is cultured and purified through brain heart infusion agar; the treatment in the first step and the second step is performed; 5, the enterococcus mass spectrum diagram to be detected is subjected to clustering analysis. The method disclosed by the method has the advantages that the operation is simple and convenient; the speed is high; the accuracy is high.

Description

technical field [0001] The invention relates to the field of bacteria detection, and in particular discloses a method for identifying clinically infected Enterococcus suis using MALDI-TOF MS technology. Background technique [0002] Enterococcus is a Gram-positive facultative anaerobic bacterium, and it is one of the common bacteria that contaminate animal food. Recently, reports on the pathogenicity of Enterococcus to animals have been increasing, especially in pigs aged 20 to 70 days. Infectious diseases characterized by swelling of subcutaneous and solid organs and hemorrhage are common in free-range and small-scale pig herds, causing serious economic losses to the pig industry. Because enterococci have different degrees of resistance to existing antibiotics, and resistance genes are transmitted from animals to humans or among other bacteria through the food chain, it is particularly important to quickly and accurately identify enterococci, and to prevent infection. The ...

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Application Information

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IPC IPC(8): G01N27/62
CPCG01N27/62
Inventor 王海花张晓峰王金合苗丽黄甫和平白建
Owner HENAN UNIV OF ANIMAL HUSBANDRY & ECONOMY
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