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Preparation method of high-strength collagen membrane having curvature

A collagen film, high-strength technology, applied in the field of collagen film, to save production time, simplify the production process, and save costs

Active Publication Date: 2017-09-29
JINAN UNIVERSITY
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

The application of traditional CV on the cornea is relatively limited, because CV is flat, while the cornea is spherical with a certain curvature

Method used

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  • Preparation method of high-strength collagen membrane having curvature

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Embodiment 1

[0024] Preparation of collagen gel: Add 9ml of 5mg / ml collagen solution into a centrifuge tube, then add 1ml of 10×DMEM medium, shake and mix well. After centrifuging at 2000rpm for 2min, put the centrifuge tube in an ice bath to prevent the collagen gel from agglomerating. Carefully suck out the liquid with a pipette and place it in a 6cm Petri dish, taking care not to generate air bubbles. Place the dish in a 37°C incubator and incubate for 30min.

[0025] Control the pressure to carry out high-concentration collagen gel compression dehydration and molding: take the collagen gel in the previous step out of the dish, place the collagen gel 400 in the nylon ring 300, and then place the collagen gel 400 flat on the lower mold 200 (The lower mold 200 includes a concave portion 210 with curvature), and then the upper mold 100 is placed on the collagen gel 400 (the upper mold 100 includes a protrusion 110 with a curvature that is compatible with the concave portion 210), as figu...

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Abstract

The invention relates to a collagen membrane, and discloses a preparation method of a high-strength collagen membrane having curvature. The method comprises the following steps: S1, preparing a collagen solution into collagen gel; S2, placing the collagen gel prepared in the step S1 into an annular fixing ring; S3, placing the annular fixing ring and the collagen gel in the annular fixing ring on a lower mold, and compressing the collagen gel with an upper mold, wherein the lower mold comprises a concave part with a curvature, the upper mold comprises a projecting part matched with the concave part with the curvature, and compression of the collagen gel is realized through cooperation of the projecting part and the concave part; S4, after finishing compression, taking the lower mold out, and drying the collagen membrane attached to the upper mold by blowing to obtain the high-strength collagen membrane having curvature. Through a method for compressing the collagen gel, moisture is drained rapidly, preparation time is saved, the prepared collagen membrane having curvature has high strength can be matched with a curved cornea well, the efficiency of corneal injury repairing is increased, and the refractive of the cornea is not influenced.

Description

technical field [0001] The invention relates to a collagen film, in particular to a preparation method of a high-strength curvature collagen film. Background technique [0002] The extracellular matrix is ​​an indispensable part of organisms. Its function is to gather various tissue cells together to form an organic whole, and at the same time maintain the microenvironment for cell growth. In the human body, the extracellular matrix plays a role in maintaining the integrity of tissues and organs such as skin, bones, cornea, tendons, teeth, and cartilage. Collagen, as the main structural protein of the extracellular matrix, plays a very important role in the living body. Collagen has a higher content in the extracellular matrix, so that it can afford a relatively large mechanical strength. [0003] As a kind of extracellular matrix, collagen has excellent biocompatibility. Collagen membrane is a good tissue engineering tool. It has the characteristics of toughness, transpa...

Claims

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Application Information

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Patent Type & Authority Applications(China)
IPC IPC(8): A61L27/24A61L27/50
CPCA61L27/24A61L27/50A61L2430/16
Inventor 陈建苏崔泽凯张军
Owner JINAN UNIVERSITY
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