Biological agent capable of improving soil environment and method for improving soil environment through same
A biological preparation, soil improvement technology, applied in the direction of microorganism-based methods, biochemical equipment and methods, microorganisms, etc., can solve problems such as decline in yield and economic benefits
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[0018] In order to make the purpose, technical solution and advantages of the present invention clearer, the present invention will be further described in detail below.
[0019] The embodiment of the present invention improves the cultivation method of soil environment biological agent, comprises the following steps:
[0020] (1) Treat gigasporarosea, gigasporasp, glomus mosseae, and glomus sp separately, sterilize the surface of a single spore, and then transfer it to a petri dish with a diameter of 5 cm; add 10 ml of 10mM MES buffer liquid, 0.04 g of Gel-Gro;
[0021] (2) Adopt a clover host, a mixed medium with a volume ratio of vermiculite and perlite of 1:1 as a matrix, wherein the diameter of the matrix particles is about 0.65 mm, and the pH is controlled between pH 5 and 7; after growing for two weeks, put the host and the matrix Transfer to the surface of the Gel-Gro medium with germinated spores together; all hosts form mycorrhiza with single germinated spores respe...
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