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Biological agent capable of improving soil environment and method for improving soil environment through same

A biological preparation, soil improvement technology, applied in the direction of microorganism-based methods, biochemical equipment and methods, microorganisms, etc., can solve problems such as decline in yield and economic benefits

Inactive Publication Date: 2017-09-26
徐晨斐
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

However, these two methods are both avoiding reality and ignoring reality. Although the soil activity can be slightly restored, the yield and economic benefits have dropped significantly.

Method used

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Embodiment Construction

[0018] In order to make the purpose, technical solution and advantages of the present invention clearer, the present invention will be further described in detail below.

[0019] The embodiment of the present invention improves the cultivation method of soil environment biological agent, comprises the following steps:

[0020] (1) Treat gigasporarosea, gigasporasp, glomus mosseae, and glomus sp separately, sterilize the surface of a single spore, and then transfer it to a petri dish with a diameter of 5 cm; add 10 ml of 10mM MES buffer liquid, 0.04 g of Gel-Gro;

[0021] (2) Adopt a clover host, a mixed medium with a volume ratio of vermiculite and perlite of 1:1 as a matrix, wherein the diameter of the matrix particles is about 0.65 mm, and the pH is controlled between pH 5 and 7; after growing for two weeks, put the host and the matrix Transfer to the surface of the Gel-Gro medium with germinated spores together; all hosts form mycorrhiza with single germinated spores respe...

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PUM

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Abstract

The invention discloses a culture method of a biological agent capable of improving the soil environment. The method comprises the following steps that strains of gigasporarosea, gigasporasp, glomus mosseae and glomussp are subjected to treatment respectively, single spore surface sterilization is performed, then, the strains are transferred into a culture dish, and an MES buffer solution and Gel-Gro are added; clover hosts are adopted, a mixture medium with vermiculite and perlite is adopted as a matrix, wherein the volume ratio of vermiculite to perlite is 1:1; the hosts and the medium are transferred to the surface of Gel-Gro medium with germinated spores after growing for two weeks; all the hosts form mycorrhizae with single germination spores respectively; single-cell fungi are obtained; wood bits are packaged into bags made of gauze and put into a bacterium solution with the fungi to be sufficiently soaked; two fungus bags are mixed with one ton of water, the weight of each bag is one kilometer, the fungus bags are placed into a ton bucket and are not dispersed, a circulating pump is used for sufficient convection, the fungus bags are taken out after 6 hours, 50 KG of fermented sheep manure is added, stirring is performed inside the ton bucket with a stirrer, standing is performed after one hour for precipitation, and two thirds of the upper portion is taken out after 24 hours to obtain the finished biological agent.

Description

technical field [0001] The invention relates to the field of biological agents, in particular to a biological agent for improving soil environment and a method for using the same to improve soil environment. Background technique [0002] Now there is a problem called "continuous cropping obstacle" in the domestic agricultural field, that is, continuous farming obstacle. It is specifically manifested in the cultivation of high value-added crops, such as watermelons, melons, and strawberries. The expression is that if the crop is planted in the Nth year, the crop cannot be planted in the N+1 year. If the crop is forced to be planted, the yield will be greatly reduced. This situation will become more and more serious over time. For example, when the In N+3 years, the output may be reduced to about 30% of the original. [0003] There are two root causes of this problem, one is that too much fertilizer causes soil compaction, and the other is the residue of crop disease bacteri...

Claims

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Application Information

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IPC IPC(8): A01B79/00A01B79/02C09K17/14C12N1/14C12R1/645C09K101/00
CPCA01B79/00A01B79/02C09K17/14C09K2101/00C12N1/14
Inventor 徐晨斐
Owner 徐晨斐
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