Pharmaceutical composition for preventing or treating cancer containing extracellular polysaccharide produced by ceriporia lacerata as active ingredient
A technology for tearing C. cereus and exopolysaccharides, which can be applied to medical preparations containing active ingredients, drug combinations, organic active ingredients, etc., and can solve the problem that the effect of preventing or treating cancer has not been reported yet.
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manufacture example 1
[0063] Production Example 1. Production of Mycelia Culture Liquid
[0064] The mucormyces cultured by subculturing the Mucoralus cereus separated from live tissues of oak trees collected in Sangju, Gyeongsangbuk-do (Korea) were frozen and stored at -80°C, and the stored strains were stored at -80°C. PDA (Potatodextrose agar) medium (87 plastic Petri dishes; culture medium (Difco), Becton Dickinson Company (Becton Dickinson and Company)) after passage 2 to 3 times, strain (hereinafter referred to as "PDA culture strain") Store in a 4°C refrigerator before use. And after forming PDB (Potato dextrose broth) medium (Difco, Becton Dickinson and Company) 600ml in Erlenmeyer flask, put a PDA culture strain here, and carry out the shock culture of eight days at 25 ℃, thus obtained PDB culture strains.
[0065]In addition, for the cultivation of the strain, the liquid culture medium was used in an 800l fermenter at 1.5kgf / cm 2 After injecting air at 121°C and sterilizing for 20 minu...
manufacture example 2
[0066] Production example 2. Production of dry powder of the culture solution of Ceruleus spp.
[0067] Using a vacuum freeze dryer, the mycelia culture solution of Ceruleus cerevisiae produced in Production Example 1 was subjected to vacuum freeze-drying at 25° C. for 72 hours to make it powder, thus producing cervus cerevisiae Dry powder of fungus mycelium culture fluid.
manufacture example 3
[0068] Production example 3. Production of the culture solution extract of Ceruleus lamina
[0069] 100 ml of distilled water was added to 5 g of the dry powder of the culture solution of the cerebrospora mycelium produced in the above-mentioned production example 2, and after suspending sufficiently, centrifugation was performed at 8,000 rpm for 20 minutes, and the supernatant Add ethanol equivalent to 2 to 3 times the amount, and let it stand at 4°C for 12 hours. Thereafter, the supernatant was extracted from the standing matter to produce an extract of the culture solution of the mycelia of C. cerevisiae.
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