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Functional linked marker 0707-1 and application thereof to germplasm improvement of maize

A corn, 27-kd technology, applied in the field of botany and molecular biology, can solve the obstacles of screening high-quality protein corn, trouble corn breeders and geneticists and other problems

Active Publication Date: 2017-09-19
CAS CENT FOR EXCELLENCE IN MOLECULAR PLANT SCI
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

[0006] However, what exactly is the high-quality protein corn endosperm modifier? What is its mechanism of action? These are still difficult problems for corn breeders and geneticists, and hinder the selection of high-quality protein corn in agriculture

Method used

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  • Functional linked marker 0707-1 and application thereof to germplasm improvement of maize
  • Functional linked marker 0707-1 and application thereof to germplasm improvement of maize
  • Functional linked marker 0707-1 and application thereof to germplasm improvement of maize

Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1、27-k

[0104] The dose effect of embodiment 1, 27-kDγ-zein protein modification factor

[0105] During the fertilization process of corn endosperm, 2 parts of its endosperm genotype are derived from the female parent, and 1 part is derived from the male parent. Therefore, the present invention utilizes K0326Y (a high-quality protein corn derived from Africa, containing 27-kDγ-zein modifier) ​​and conventional maize inbred line B73 (without 27-kDγ-zein modifier) ​​were designed to detect the genetic expression pattern of its 27-kDγ-zein modifier.

[0106] The results showed that the expression of 27-kDγ-zein modifier in the endosperm of homozygous inbred line K0326Y was the highest at 3 doses, and then the expression of K0326Y was the female parent (2 doses) and B73 was the male parent (0 dose). B73 is the female parent (0 dose) K0326Y is the male parent (1 dose) decreased in turn, and finally the B73 homozygous inbred line (0 dose) had the lowest expression level, as shown in figur...

Embodiment 2、27-k

[0107] Example 2, 27-kDγ-zein protein modifier is a cis-acting element

[0108] Modifiers include cis-acting or trans-acting elements, wherein cis-acting elements refer to specific DNA sequences that have transcriptional regulation functions in the same DNA molecule. Including promoters, enhancers, etc. Trans-acting factors refer to proteins that can directly or indirectly recognize or bind to the core sequences of various cis-acting elements and participate in regulating the transcription efficiency of target genes, most of which are transcription factors.

[0109] In order to verify the mode of action of the 27-kDγ-zein modifier, the inventors found a special inbred line XF134 among hundreds of common maize inbred lines. 18bp difference ( image 3 B), so its encoded protein sequence has a length polymorphism with common inbred lines ( image 3 A). Therefore, after crossing XF134 with B73 and K0327Y, the grain endosperm of 18 days of pollination was extracted, the 27kd ge...

Embodiment 3、27-k

[0111] Example 3, 27-kDγ-zein protein modifier association and linkage population construction

[0112] By collecting polymorphic germplasm resources from tropical and temperate regions, the location analysis of 27-kDγ-zein modifiers can be carried out by GWAS and QTL mapping methods. Finally, the inventor collected 492 maize inbred lines and 280 IBM Syn10 haploid populations for analysis, and there was a significant difference in the expression of 27-kDγ-zein gene. Some results are as follows Figure 4 A, 4B.

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Abstract

The invention relates to a functional linked marker 0707-1 and application thereof to the germplasm improvement of maize. In the functional linked marker 0707-1, one major modifying factor of 27-kD[gamma]-zein is positioned for the first time, is located in the range of 100kb near of the 120Mb of a No. 7 chromosome, and is the multicopy of the 27-kD[gamma]-zein. According to the application, a molecular marker and an identification method for identifying a gene of the multicopy are also established; therefore, an effective approach is provided for identifying quality protein maize.

Description

technical field [0001] The invention belongs to the fields of botany and molecular biology, and more specifically, the invention relates to a functional linkage marker 0707-1 and its application in maize germplasm improvement. Background technique [0002] High-quality protein maize (Quality Protein Maize, QPM) is a high-lysine corn with nutritional value comparable to that of milk, and its yield is close to or even higher than that of ordinary corn. Malnutrition caused by lack of lysine and tryptophan in national population. Cultivating high-quality protein corn will greatly improve the quality and benefit of corn kernels, and comprehensively increase farmers' income. However, high-quality protein corn is improved on the background of tropical germplasm resources, and it shows good hard endosperm kernels in tropical areas. After being introduced into China, tropical germplasm often exhibits poor agronomic traits such as delayed growth period, soft or semi-soft grain, into...

Claims

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Application Information

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Patent Type & Authority Applications(China)
IPC IPC(8): C12Q1/68C12N15/11
CPCC12Q1/6895C12Q2600/13C12Q2600/158
Inventor 巫永睿刘红军孙传龙
Owner CAS CENT FOR EXCELLENCE IN MOLECULAR PLANT SCI
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