A method for rapid determination of tocopherol content in Brassica napus seeds using near-infrared spectroscopy

Abstract

The invention relates to a method of quickly determining tocopherol content in cabbage type rape seeds by using a near infrared spectrum. The method specially comprises the following steps: collecting spectral data of a sample by using an infrared spectrometric analyzer as a correcting set; pre-processing the near infrared spectrum of the correcting set; associating the chemical value of the sample of the correcting set with the data of the near infrared spectrum to establish different calibrating equations and determining a correcting model; and performing external verification on the near infrared correcting model by using a verifying set. The method provided by the invention has the advantages that the tocopherol content in the cabbage type rape seeds can be quickly and efficiently determined without loss, and a convenient and effective method for breeding rape with high tocopherol is provided, so that the breeding process of a novel variety is extremely accelerated.

Description

technical field [0001] The invention relates to the field of crop quality breeding, in particular to a method for rapidly determining tocopherol content in Brassica napus seeds by using near-infrared spectroscopy. Background technique [0002] Vitamin E, including tocopherols and tocotrienols, is an important fat-soluble vitamin with strong antioxidant properties and plays an irreplaceable role in animal and plant life. Natural vitamin E is mainly synthesized in the seeds and leaves of oil crops. Rape is a dicotyledonous plant and only contains tocopherols. Because rapeseed seeds are rich in tocopherols and have a large planting area, tocopherol breeding in rapeseed has become one of the hotspots of research and has attracted more and more attention from breeders. The determination of tocopherol content in rapeseed is mainly measured by liquid chromatography (HPLC), which is generally complicated. It consumes seeds and uses a lot of chemical reagents. No matter in terms of ...

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Problems solved by technology

The determination of tocopherol content in rapeseed is mainly carried out by liquid chromatography (HPLC), which is generally more complicated. It not only consumes seeds, but also uses a large amount of chemical reagents, no matter in terms of time, funds or environmental protection. It is necessary to find more convenient and effective methods

[0003] Near-infrared spectroscopy is a new technology that has gradually attracted attention in the analysis and testing industry in recent years. With its fast, efficient and non-destructive detection advantages, it has been widely used in the field of crops, but the use of near-infrared spectroscopy to determine rape or other crops The method of seed tocopherol content has not been reported so far. Although it has been used in the detection of vitamin E content in feed, the model established in feed or even the analysis model established with other seeds is completely unsuitable for the determination of vitamin content in rapeseed. Yes, this is mainly because the chemical composition of different crop seeds is different, and the double frequency and combined frequency vibration frequencies of hydrogen-containing groups are different, so the peak position, peak number and peak intensity of the near-infrared spectrum are also different, that is, the spectra of different substances are If there is specificity, then the model established with rapeseed is only applicable to rapeseed, so the method of measuring tocopherols in rapeseed must be established

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