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SNP (single nucleotide polymorphism) molecular markers closely linked to pumpkin lutein content major QTL (quantitative trait loci) and application of SNP molecular markers

A molecular marker, lutein technology, applied in recombinant DNA technology, microbial determination/inspection, DNA/RNA fragments, etc., can solve the problems of time-consuming and labor-intensive, disadvantageous to pumpkin breeding, and achieve the effect of shortening time

Active Publication Date: 2017-09-08
INST OF VEGETABLES GUANGDONG PROV ACAD OF AGRI SCI
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Problems solved by technology

Although it is feasible to breed high-lutein varieties by traditional breeding methods, it is time-consuming and labor-intensive, which is not conducive to pumpkin breeding in my country

Method used

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  • SNP (single nucleotide polymorphism) molecular markers closely linked to pumpkin lutein content major QTL (quantitative trait loci) and application of SNP molecular markers
  • SNP (single nucleotide polymorphism) molecular markers closely linked to pumpkin lutein content major QTL (quantitative trait loci) and application of SNP molecular markers
  • SNP (single nucleotide polymorphism) molecular markers closely linked to pumpkin lutein content major QTL (quantitative trait loci) and application of SNP molecular markers

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Embodiment Construction

[0029] The SNP molecular marker closely linked to the main effect QTL locus of pumpkin lutein content is molecular marker 6564 or molecular marker 24019, which is characterized in that the molecular marker is located on the linkage group No. Both sides of the gene; wherein, molecular marker 6564 contains the nucleotide sequence shown in SEQ ID NO.1 or its homologous SNP sequence; molecular marker 24019 contains the nucleotide sequence shown in SEQ ID NO.2 or its homologous SNP sequence.

[0030] Further, the 114th base from the 5' end of the sequence shown in SEQ ID NO.1 is a SNP site, and its base is T or C. When the SNP site has only one base in the two alleles as T, the pumpkin lutein gene closely linked to the molecular marker 6564 is identified as high lutein content in the fruit, when the SNP site is in When the bases in the two alleles are both C, the fruit is pumpkin with low lutein content.

[0031] Further, the 24th base from the 5' end of the sequence shown in SEQ...

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Abstract

The invention discloses SNP (single nucleotide polymorphism) molecular markers closely linked to pumpkin lutein content major QTL (quantitative trait loci) and application of the SNP molecular markers. The molecular markers are positioned on a Chinese pumpkin No. 11 linkage group and are respectively located on two sides of a lutein gene, wherein the molecular marker 6564 comprises a nucleotide sequence as shown in SEQ ID No. 1 or the homologous SNP sequence of the nucleotide sequence as shown in SEQ ID No. 1; the molecular marker 24019 comprises a nucleotide sequence as shown in SEQ ID No. 2 or the homologous SNP sequence of the nucleotide sequence as shown in SEQ ID No. 2. The SNP molecular markers have the advantages that the positioned QTL is significantly correlated to quantitative traits (p<0.05) and is high in contribution rate, the explanation probability of the molecular marker 6564 is 15.2%, the explanation probability of the molecular marker 24019 is 15.6%, and pumpkin lutein content can be predicted through the two molecular markers; dCAPS (derived cleaved amplified polymorphic sequences) amplification primers designed according to the molecular markers can be applied to pumpkin variety improvement molecular marker assisted selection in a simple, fast and high-throughput manner, molecular marker assisted selection technical support is provided for the early identification and screening of pumpkin lutein content traits, and the time of traditional gene mapping is shortened greatly at the same time.

Description

technical field [0001] The invention belongs to the field of molecular detection technology and breeding, in particular to a SNP molecular marker closely linked with the main effect QTL of pumpkin lutein content and its application. Background technique [0002] Lutein is a natural substance that widely exists in vegetables, flowers, fruits and other plants. Natural lutein is an excellent antioxidant that can resist cell and organ damage caused by free radicals in the human body, thereby achieving Prevent cardiovascular sclerosis and coronary heart disease caused by aging of the body, and at the same time prevent power decline and blindness caused by senile macular degeneration. [0003] Pumpkin is rich in carotenoids, especially rich in lutein, with 0-17mg of lutein per 100g fresh product. With the deepening of scientific research and the needs of society, people pay more and more attention to the nutritional and health effects of food. Therefore, breeding pumpkins with hi...

Claims

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Application Information

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IPC IPC(8): C12Q1/68C12N15/11
CPCC12Q1/6895C12Q2600/156
Inventor 钟玉娟黄河勋罗少波李俊星周洋洋谢大森
Owner INST OF VEGETABLES GUANGDONG PROV ACAD OF AGRI SCI
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