The use of kits and reagents in the preparation of kits
A kit and technology for use, applied in a kit for diagnosing acute coronary syndrome, and the reagent in the field of kit preparation
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Embodiment 1
[0046] Example 1 Differential expression profile of miRNAs 1h after plaque rupture compared to before rupture
[0047] The baseline data of all selected patients are shown in Table 1. Plasma samples were collected from 10 patients before balloon expansion (that is, before plaque rupture) and 1 hour after balloon expansion (that is, after plaque rupture). After RNA was extracted, microarray detection was performed. A total of 24 miRNAs were differentially expressed 1 h after plaque rupture, 7 up-regulated miRNAs, and 14 down-regulated miRNAs, as shown in Table 2.
[0048] Table 1: Baseline data of the study population
[0049]
[0050] Table 2: Differential expression of miRNAs after plaque rupture
[0051]
[0052] The above are the miRNAs with differential expression fold (fold change)>2 times and false discovery rate (false discovery rate)<0.0001% between the two groups
Embodiment 2
[0053] Example 2 Verification of 24 differentially expressed miRNAs in the chip above:
[0054] In the other 10 patients, plasma samples were collected before balloon dilatation (that is, before plaque rupture) and 1 hour after balloon dilation (that is, after plaque rupture). After RNA was extracted, real-time PCR was used to detect The expression levels of 24 miRNAs were screened, and 8 miRNAs were still differentially expressed, including miR-155-5p, miR-212-3p, miR-483-5p, miR-1233-3p, miR-20b-5p, miR-122-5p, miR-451a, miR-486-5p, see figure 1 . In addition, because the expression abundance of miR-376a is very low, it is almost undetectable in the first three, so the follow-up verification was abandoned.
[0055] Since the collected specimens before and after balloon dilation contained contrast agents, in order to eliminate the influence of contrast agents on miRNA levels, the inventors collected plasma from 10 patients before and after contrast, and detected the above-m...
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