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A DNA molecular logic gate based on dna Origami and its construction method

A technology of DNA molecules and logic gates, applied in the field of DNA molecular logic gates based on DNAOrigami and its construction, can solve problems such as hindering the development of sensing systems and difficult logic operations

Active Publication Date: 2019-11-12
NORTH CHINA ELECTRIC POWER UNIV (BAODING)
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

In addition, in most previous nanosystems assembled with nanoparticles and DNA origami, it is very difficult to realize the logic operation of multiple inputs at the same time, which greatly hinders the development of DNA origami-based sensing systems.

Method used

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  • A DNA molecular logic gate based on dna Origami and its construction method
  • A DNA molecular logic gate based on dna Origami and its construction method
  • A DNA molecular logic gate based on dna Origami and its construction method

Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0060] Example 1: Two-Input OR (OR) Logic Gate

[0061] (1) 1.6nM M13mp18 chain (circular chain of about 7000 bases) and 202 short staple chains (Table 1) were mixed in 1×TAE / Mg2+ buffer at a ratio of 1:10, and from 90 After annealing to room temperature, it is assembled into a DNA Origami-1 template, and three helper chains a1, b1, and c1 are extended from a specific position on the surface of the DNA Origami-1 template;

[0062] (2) The thiolated DNA strand a1' is combined with 15nm gold particles (AuNP) to form DP-A conjugates; the thiolated DNA strands b1' and c1' are combined with 5nm gold particles (AuNP) respectively to form DP-B and DP-C conjugates; wherein, chains a1', b1', c1' can recognize helper chains a1, b1, c1, respectively;

[0063] (3) DNA Origami-1 template in step (1) hybridizes with DP-B and DP-C conjugates in step (2) to form AuNP / origami conjugates (product I), such as figure 1 a;

[0064] (4) A DNA input strand is added to step (3), and a logical oper...

Embodiment 2

[0068] Example 2: Two-Input AND (AND) Logic Gate

[0069] (1) with the steps in Example 1, the difference is that the assembly is a DNA Origami-2 template, and 3 helper chains a1, d1, e1 are extended from a specific position on the surface of the DNA Origami-2 template;

[0070] (2) The thiolated DNA strand a1' is combined with 15 nm gold particles (AuNP) to form DP-A conjugates; The molar ratios combine to form DP-DE-1 conjugates; wherein the chains a1', d1', and e1' can recognize the helper chains a1, d1, and e1, respectively;

[0071] (3) The DNA Origami-2 template in step (1) hybridizes with the conjugate of DP-A and DP-DE-1 in step (2), and the conjugate NP-DE-1 and origami-2 pass through the chain d1' / e1' and d1 / e1 are hybridized with each other, and the reference NP-A is also connected to the surface of origami-2, forming AuNP / origami conjugates (product V) such as figure 2 a;

[0072] (4) A DNA input strand is added to step (3), and a logical operation is realized ...

Embodiment 3 3

[0078] Embodiment 3 Three-input AND (AND) logic gate

[0079] (1) 1.6nM M13mp18 chain (circular chain of about 7000 bases) and 202 short staple chains were mixed in 1×TAE / Mg2+ buffer at a ratio of 1:10, and annealed from 90°C to room temperature , assembled into a DNA Origami-3 template, and two helper chains f1, g1 extend from a specific position on the surface of the DNA Origami-3 template;

[0080] (2) The thiolated DNA strand f1', the thiolated DNA strand T1 and the 15 nm gold particles AuNP form a conjugate NP-M in a molar ratio of 1:1:1; the thiolated DNA strand g1', the thiolated DNA strand T2 Form a conjugate NP-N with 15nm gold particles AuNP in a molar ratio of 1:1:1;

[0081] (3) DNA Origami-3 template in step (1), DP-M, DP-N conjugate in step (2) hybridize with bridge chain T to form AuNP / origami conjugate (product VII);

[0082] (4) A DNA input strand is added to step (3), and a logical operation is realized by selectively releasing AuNPs using strand displaceme...

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Abstract

The invention belongs to the field of molecular computation, and particularly relates to a DNA molecular logic gate based on DNA Origami and a construction method thereof. The logic gate comprises an input signal, a signal converter and an output signal; the signal converter includes a DNA Origami template, a helper chain positioned on the DNA Origami template, and a DNA / AuNP conjugate that can be identified and connected with the helper chain; and the output signal is provided for releasing the DNA / AuNP conjugate from the DNA Origami template. According to the DNA molecular logic gate based on the DNA Origami and the construction method thereof disclosed by the invention, the selective and dynamic release to gold particles is implemented by a special DNA signal on the DNA Origami, and thus, the operation of the logic gate is implemented; moreover, by employing a gold particle dimer connected by the DNA chain, the operation of simultaneously imputing three input chains for the logic gate is implemented. The DNA molecular logic gate and the construction method thereof provide a concept to construct complex molecular circuit and nano device; and through combining with various nano engineering strategies, the DNA molecular logic gate and the construction method thereof can be used for assembling a large programmable structure, and also pave a road for sensitivity detection of various molecular conformations.

Description

technical field [0001] The invention belongs to the field of molecular computing, in particular to a DNA molecular logic gate based on DNA Origami and a construction method thereof. Background technique [0002] In recent years, DNA self-assembly has become a powerful tool for building nanostructures, with a wide range of applications in building a wide variety of 2D and 3D structures, including planar arrays, cylindrical tubes, and various geometries. DNAorigami was first discovered by Rothemund, who formed preliminary nanostructures by hybridizing a long DNA single strand M13 with hundreds of short DNA helper strands, providing researchers with a highly efficient DNA nanostructure with well-defined shape and size. method of structure. Now, DNA origami has been applied in various fields such as molecular signal sensing and logic computing. Gold particles have unique structural and optical properties, and a method that can fully control the assembly of gold particles with ...

Claims

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Application Information

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Patent Type & Authority Patents(China)
IPC IPC(8): H03K19/20G06N3/00
CPCG16B25/00H03K19/20
Inventor 杨静张成吴燃峰宋智超
Owner NORTH CHINA ELECTRIC POWER UNIV (BAODING)
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