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A kind of culture medium suitable for Clostridium difficile and preparation method thereof

A technology for Clostridium difficile, culture medium, applied in microorganism-based methods, biochemical equipment and methods, bacteria, etc., and can solve problems such as difficult growth

Active Publication Date: 2018-04-13
广西华银医学检验所有限公司 +1
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

[0004] The prior art shows that this bacterium is a strictly obligate anaerobic bacterium, and it is not easy to grow by conventional anaerobic culture methods.

Method used

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  • A kind of culture medium suitable for Clostridium difficile and preparation method thereof
  • A kind of culture medium suitable for Clostridium difficile and preparation method thereof

Examples

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Comparison scheme
Effect test

Embodiment 1

[0014] Example 1 The present invention is suitable for the culture medium of Clostridium difficile

[0015] A medium suitable for Clostridium difficile, which is prepared from the following components by weight: 10 parts of peptone, 6 parts of dehydrated calf brain powder, 10 parts of dehydrated beef heart powder, 1.0 part of glucose, and 0.1 part of magnesium sulfate Parts, 1.5 parts of laminine, 0.01 parts of natamycin, 0.5 parts of sodium dihydrogen phosphate, 0.5 parts of glycine, 10.3 parts of vitamin B, 800 parts of purified water, pH adjusted to 7.2.

[0016] The preparation method of the culture medium is as follows: Measure one-half of the prescription amount of purified water, add peptone, dehydrated calf brain extract powder, dehydrated beef heart extract powder and glucose to it, stir to dissolve it, and add the remaining purified water , Add magnesium sulfate and sodium dihydrogen phosphate to it, adjust its pH to 7.2, sterilize at 121°C for 20 minutes, add glycine, la...

Embodiment 2

[0017] Example 2 The present invention is suitable for the culture medium of Clostridium difficile

[0018] A culture medium suitable for Clostridium difficile, which is prepared from the following components by weight: 15 parts of peptone, 8 parts of dehydrated calf brain powder, 15 parts of dehydrated beef heart powder, 1.5 parts of glucose, and 0.5 parts of magnesium sulfate Parts, 2.5 parts of laminine, 0.03 parts of natamycin, 0.9 parts of sodium dihydrogen phosphate, 0.9 parts of glycine, 10.5 parts of vitamin B, 1000 parts of purified water, pH adjusted to 7.4.

[0019] The preparation method of the culture medium is as follows: Measure one-half of the prescription amount of purified water, add peptone, dehydrated calf brain extract powder, dehydrated beef heart extract powder and glucose to it, stir to dissolve it, and add the remaining purified water , Add magnesium sulfate and sodium dihydrogen phosphate to it, adjust its pH to 7.4, sterilize at 121°C for 20 minutes, add ...

Embodiment 3

[0020] Example 3 The present invention is suitable for the culture medium of Clostridium difficile

[0021] A medium suitable for Clostridium difficile, which is prepared from the following components by weight: 12.5 parts of peptone, 7 parts of dehydrated calf brain powder, 12.5 parts of dehydrated beef heart powder, 1.25 parts of glucose, and 0.3 parts of magnesium sulfate Parts, 2 parts laminine, 0.02 parts natamycin, 0.7 parts sodium dihydrogen phosphate, 0.7 parts glycine, 10.4 parts vitamin B, 900 parts purified water, adjusted to pH 7.3.

[0022] The preparation method of the culture medium is as follows: Measure one-half of the prescription amount of purified water, add peptone, dehydrated calf brain extract powder, dehydrated beef heart extract powder and glucose to it, stir to dissolve it, and add the remaining purified water , Add magnesium sulfate and sodium dihydrogen phosphate to it, adjust its pH to 7.3, sterilize at 121°C for 20 minutes, add glycine, laminine and vi...

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Abstract

The invention relates to a culture medium suitable for clostridium difficile, and a preparation method thereof, and belongs to the technical field of microbial cultivation. The preparation method is used for solving a problem in the prior art that culturing effect of conventional culture medium on clostridium difficile is poor. The culture medium comprises composite ingredients such as peptone and dehydrated beef heart infusion, and also comprises complement components such as glycine and laminine. It is shown by experiment results that culture effect of the culture medium on clostridium difficile is excellent, and the culture medium possesses important implication in study on the biological characteristics of clostridium difficile and on treatment of clostridium difficile infection.

Description

Technical field [0001] The invention relates to a culture medium suitable for Clostridium difficile and a preparation method thereof, and belongs to the technical field of microorganism culture. Background technique [0002] Clostridium difficile (Clostridium difficile) is an obligate anaerobe of the Clostridium genus. It is very sensitive to oxygen and is difficult to isolate and cultivate, hence its name. It was discovered in 1935, but until 1977, it was discovered that this bacterium was related to pseudomembranous enteritis caused by the long-term clinical use of certain antibiotics (ampicillin, cephalosporin, erythromycin, clindamycin, etc.), and it was paid attention to. Clostridium difficile is widely distributed in natural habitats, such as soil, hay, sand, the feces of some large animals (cattle, donkey, and horse), and the feces of dogs, cats, rodents and humans. In addition, it also exists in large numbers The feces of infants in the intestines of water and animals of...

Claims

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Application Information

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Patent Type & Authority Patents(China)
IPC IPC(8): C12N1/20C12N1/04C12N1/02C12R1/145
CPCC12N1/02C12N1/04C12N1/20
Inventor 陈明慧王晓丹梁丽霞石秀娟
Owner 广西华银医学检验所有限公司
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