Method for increasing purity of crossing parents of asparagus

Abstract

The invention provides a method for increasing the purity of crossing parents of asparagus. The method comprises the following steps: measuring photosynthetic rate Pi of simulated leaves of the same parts of parent plants for preparing a hybrid of the asparagus; screening abnormal photosynthetic rate Pi' according to a screening rule, and marking the parent plants corresponding to Pi' as candidate abnormal plants; observing the agronomic characters of the candidate abnormal plants in the subsequent growth process, and clearing the abnormal plants which are greatly different from the parent plants; and observing flower organs of the parent plants in a flowering period, and clearing amphiprotic plants which simultaneously have stamens and pistils. According to the method, the candidate abnormal plants are recognized from a parent plant colony by virtue of the difference of the photosynthetic rates of the asparagus plants, and the abnormal plants are identified according to the features such as the plant height, the density and green degree of simulated leaves and the like; and the amphiprotic plants are identified by observing the organs in the flowering period, and the abnormal plants and the amphiprotic plants are accurately identified and timely cleared, so that the purity of the crossing parents of the asparagus can be rapidly increased, the blending of hybridization breeding is effectively avoided, and the purify of hybrid seeds of the asparagus is substantially increased.

Description

technical field [0001] The present invention relates generally to the field of hybridization of asparagus, and in particular, to methods of increasing the purity of hybrid parents of asparagus. Background technique [0002] Asparagus is dioecious, and it is difficult for parents to reproduce. Generally, asparagus tissue culture is used to rapidly multiply the parents in large quantities. Theoretically, the somatic clones obtained by tissue culture techniques should be exactly the same as the original donor plants, but in fact, in the process of dedifferentiation, differentiation and formation of regenerated plants in plant isolated tissues or cell cultures, genetic variation is often phenomenon that occurs. The mutation frequency of somatic cell clones is much higher than that of natural mutations. In a tissue culture cycle, 1% to 3% of clonal mutations can be produced, and sometimes even as high as 90%. The mutation rate of a specific trait is 0.2%~ 3%. Therefore, in th...

AI Technical Summary

Problems solved by technology

Therefore, if the asparagus parents are impure before seed production, especially if there are hermaphroditic plants remaining in the male parent, it will affect the purity of hybrid seeds produced for several years.

Once the hybrid seeds with low purity are sown, it will also affect the asparagus production and economic benefits in the next 10-15 years.