Stem-tip micro-bud grafting puncture method

Abstract

The invention belongs to the field of fruit tree planting and particularly relates to a micro-bud grafting puncture method and an application thereof, in particular to a stem-tip micro-bud grafting puncture method. The stem-tip micro-bud grafting puncture method includes: taking a scion 1-2mm long and containing 3-4 leaf primordias and soaking the scion in 1mg / L 6-BA for 10 minutes for pretreatment; under aseptic conditions, topping off a stock leaving a stem 1.5-2cm in length, longitudinally cutting through the position 5-8mm from the top, allowing the cut to be through with the joint of an xylem one third of the stem section and a phloem so that I-shaped cuts are formed in two sides of the stem section, and shifting the 3-4 leaf primordias retained on the scion to the cuts. With the method, problems that traditional stem-tip micro-bud grafting methods of an inverted 'T' grafting method, a splitting grafting method, a triangle grafting method and the like are difficult in operation, large in cut, unstable in scion, prone to water loss in growth and difficult to combine with the stock fully are solved, survival rate of stem-tip micro-bud grafting is increased, production efficiency of virus-free seedlings is increased, and production efficiency for further non-toxic seedling breeding is improved.

Description

technical field [0001] The invention belongs to the field of planting, and relates to a method for grafting stem tip microbuds, in particular to a grafting and puncturing method for stem tip microbuds. Background technique [0002] With the development of modern science and technology, asexual reproduction is often used in the cultivation process of fruit trees. Although asexual reproduction can increase production efficiency, fruit trees will be cross-infected with various viral diseases and similar viral diseases during production. Many infectious diseases such as citrus huanglongbing Not only is it a devastating disease, but there is currently no drug that can inhibit the HLB virus without harming the plant itself. Therefore, it is imminent to establish a complete grapefruit virus-free seedling propagation system. At present, there are four methods for controlling virus-like viroids in fruit trees, among which shoot tip microbud grafting has been proved to be able to obt...

AI Technical Summary

Problems solved by technology

Micro-bud grafting with T” grafting method has a large wound, and is exposed to the air, so it is easy to lose water. In addition, there are disadvantages that the rootstock and scion are not stable, and the incision is easy to dry; the wound of split grafting is too large, and the scion is easy to dehydrate and die. Low survival rate; △ connection method: 45° to the stem segment, cut off on both sides, and then cross-cut once

Therefore, the procedure of the operation method is relatively complicated, the rootstock is easily damaged, and the grafting is difficult and time-consuming.

[0005] Therefore, is it possible to provide a method for grafting shoot tip microbuds to solve the problem of easy inactivation of grafted scions and unstable combination of stock and ear in conventional grafting techniques, so as to improve the survival rate of grafting, thereby solving the technical problems urgently to be solved by those skilled in the art?

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