Biological chip for carrying out screening positioning and detection on rare cells in blood

Abstract

The invention discloses a biological chip for carrying out screening positioning and detection on rare cells in blood. The biological chip comprises a base layer and a micro-fluidic passage layer. The micro-fluidic passage layer is clung to the base layer; a capture tank is formed on the upper surface of the micro-fluidic passage layer; a plurality of enrichment pits are formed at the tank bottom of the capture tank; a depth of each enrichment pit is 15 to 50 micrometers; a signal amplification layer is clung to the upper surface of the micro-fluidic passage layer; a nanogold layer is arranged at the position on the upper surface of the signal amplification layer, which corresponds to the capture tank; an inlet passage and a discharge passage are formed in a mode of running through the nanogold layer and the signal amplification signal; the inlet passage and the discharge passage are respectively communicated with the capture tank. The biological chip has the advantages that circulating tumor cells can be efficiently captured, the circulating tumor cells cannot be damaged, and the biological chip is convenient for subsequent detection.

Description

technical field [0001] The invention relates to a biological chip, in particular to a biological chip for screening, positioning and detecting rare cells in blood. Background technique [0002] In recent years, the research of CTC (Circulating Tumor Cell: Circulating Tumor Cell) has received more and more attention, and many research reports have confirmed that CTC has the advantages of early diagnosis of tumor metastasis, curative effect monitoring, individualized treatment and exploration of tumor metastasis mechanism. Potential value, however, the content of CTCs in the circulatory system is extremely low, and one or several CTCs are often found in about 10 million normal blood cells. It is extremely difficult to effectively enrich these cells, and special biochips and Sensitive detection equipment, which has become a major difficulty limiting its clinically relevant applications. [0003] Currently, the sorting and enrichment methods for CTCs are mainly divided into two...

AI Technical Summary

Problems solved by technology

The biochip with this structure has the following disadvantages during use: 1. Free nanoparticles and labeled nanoparticles tumor cells tend to be adsorbed and aggregated in a small area on the glass slide, which may affect the recognition process. 2. The labeled target nanoparticle tumor cells roll in the weak magnetic field area and can easily escape from the microfluidic channel without being captured by the magnetic body

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