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A method for fluorescence quantitative calculation based on recombinase-mediated isothermal nucleic acid amplification (RAA) method

A technology for isothermal nucleic acid amplification and fluorescence quantification, which is used in genomics, proteomics, instruments, etc.

Active Publication Date: 2020-06-05
宁波奇天基因科技有限公司
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  • Abstract
  • Description
  • Claims
  • Application Information

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Problems solved by technology

[0020] At present, there is no isothermal nucleic acid amplification technology that can be quantified. In order to better apply and promote RAA nucleic acid amplification technology, this method was invented, so that RAA technology can be applied faster, more accurately and more widely in modern agriculture, medicine, Food safety, gun inspection and quarantine, disease control and prevention, etc.

Method used

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  • A method for fluorescence quantitative calculation based on recombinase-mediated isothermal nucleic acid amplification (RAA) method
  • A method for fluorescence quantitative calculation based on recombinase-mediated isothermal nucleic acid amplification (RAA) method
  • A method for fluorescence quantitative calculation based on recombinase-mediated isothermal nucleic acid amplification (RAA) method

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Embodiment 1

[0058] Embodiment 1: Taking yellow fever virus unknown concentration RNA as an example to further illustrate the present invention.

[0059] The present embodiment carries out according to the following steps:

[0060] 1. Source of samples with unknown copy number and yellow fever virus RNA extraction

[0061] The virus sample was the live attenuated yellow fever vaccine (Beijing Tiantan Biology) provided by Zhejiang International Travel Health Care Center, and the RNA extraction was carried out using a thermal extraction kit AM1836 (ambion, life technologies), and the extraction equipment was KINGFISHER automatic nucleic acid extraction instrument ( Thermo), carry out nucleic acid extraction and obtain yellow fever virus RNA according to above specification and operation method.

[0062] 2. The source of RAA yellow fever fluorescence detection kit

[0063] The RAA yellow fever fluorescence detection kit is provided by Jiangsu Qitian Gene Dust Technology Co., Ltd. The operat...

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Abstract

The invention discloses a fluorescent quantitation calculation method based on a recombinase-aid isothermal nucleic acid amplification (RAA) method. A set of mathematical models and calculation methods are established for the RAA nucleic acid amplification technical features, quantitative detection can be conducted accurately to expand the initial template quantity, the RAA nucleic acid amplification technology is better applied and popularized, and the RAA technology is more rapidly, accurately and widely applied to detection and scientific research in the fields of modern agriculture, medicine, food safety, inspection and quarantine, disease control and prevention, environmental microbiological identification and the like.

Description

technical field [0001] The invention belongs to the field of molecular biology detection, and specifically relates to a method for fluorescence quantitative calculation based on a recombinase-mediated isothermal nucleic acid amplification (RAA) method, which can accurately quantify the starting amount of nucleic acid amplification. Background technique [0002] In biological research and molecular diagnosis, DNA amplification is a basic and necessary technical means, the most commonly used of which is the use of polymerase chain reaction (English full name: Polymerase Chain Reaction, referred to as PCR) for in vitro nucleic acid amplification, This technology was invented in 1983 by Dr. Mullis of the Genetics Department of the American scientist PE (Perkin Elmer Perkin-Elmer). The Bell Prize has been widely used in modern agriculture, medicine and food industry. Through this technology, the efficient amplification of trace amounts of nucleic acid can be achieved, and a very...

Claims

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Application Information

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Patent Type & Authority Patents(China)
IPC IPC(8): G16B20/10
CPCG16B20/00
Inventor 应清界应若晨郭利川王秀东王智宏
Owner 宁波奇天基因科技有限公司
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