gnrh antigen and its application in the castration effect and meat quality of bulls by active immunization
A product and animal technology, applied in the direction of vertebrate antigen components, medical preparations containing active ingredients, and antibody medical components, etc., can solve problems such as unsatisfactory effects and differences in immune results, and improve the recognition ability of the immune system and enhance immunity. The effect of enhancing the immunogenicity of antigens
- Summary
- Abstract
- Description
- Claims
- Application Information
AI Technical Summary
Problems solved by technology
Method used
Image
Examples
Embodiment 1
[0090] The preparation of embodiment 1, GnRH vaccine
[0091] 1. Obtaining GnRH derivatives
[0092] GnRH short peptides in nature are composed of 9 kinds of amino acids, namely glutamic acid, histidine, tryptophan, serine, tyrosine, glycine, leucine, arginine and proline. The 6th glycine in the GnRH short peptide was replaced by D-lysine, and four types of GnRH epitopes were designed, including eight GnRH derivatives shown in Table 1. The eight GnRH derivatives were: Molecular GnRH antigen tandem, double cysteine tandem, insertion of a short peptide MAL that is easy to form an α-helix, and insertion of a short peptide VIY that is easy to form a β-sheet (see Table 1 for the sequence of the antigenic epitope).
[0093] Table 1 is the amino acid sequence of GnRH derivatives
[0094]
[0095]
[0096] The eight GnRH derivatives A-H shown in the above Table 1 were synthesized by Shanghai Gil Biochemical Co., Ltd., and purified by high performance liquid chromatography (H...
Embodiment 2
[0107] Embodiment 2, the application of GnRH vaccine
[0108] 1. GnRH vaccine immunization mice small test
[0109] 1. Experimental animals
[0110] Twenty-seven healthy male mice (purchased from the Animal Experiment Center of Shihezi University) of 1 month old and with similar body weight were randomly divided into 9 groups, which were designated as the control group, group A, group B, group C, group D, and group E respectively. Group, Group F, Group G, Group H;
[0111] 2. Immunity
[0112] Immune groups A-H: at the age of 1 month, respectively take the method of leg muscle injection to the 8 groups of male mice of A-H, and correspondingly immunize 1 ml of the 8 kinds of GnRH vaccines A-H (containing about 50 μg of GnRH) obtained in Example 1; The experimental animals were boosted once with the injection method.
[0113] Control group: the experimental animals were immunized with the vaccine emulsified by physiological saline and adjuvant, and the feeding and management...
PUM
![No PUM](https://static-eureka-patsnap-com.libproxy1.nus.edu.sg/ssr/23.2.0/_nuxt/noPUMSmall.5c5f49c7.png)
Abstract
Description
Claims
Application Information
![application no application](https://static-eureka-patsnap-com.libproxy1.nus.edu.sg/ssr/23.2.0/_nuxt/application.06fe782c.png)
- R&D Engineer
- R&D Manager
- IP Professional
- Industry Leading Data Capabilities
- Powerful AI technology
- Patent DNA Extraction
Browse by: Latest US Patents, China's latest patents, Technical Efficacy Thesaurus, Application Domain, Technology Topic.
© 2024 PatSnap. All rights reserved.Legal|Privacy policy|Modern Slavery Act Transparency Statement|Sitemap