Multispectral fluorescence imaging system and method based on hard endoscope

Abstract

The invention relates to a multispectral fluorescence imaging system and method based on a hard endoscope, in order to solve the problem that the hard endoscope can only conduct excitation imaging on specific fluorescent probes in the prior art. When images of tissue to be detected are collected by the system, the end of a hard endoscope module stretches into the tissue to be detected, excitation light is emitted into the tissue to be detected through a light source control module, the excited fluorescent probes in the tissue to be detected can not only emit fluorescence signals but also reflect the excitation light, different optical filters enter light passing positions through adjusting an optical filter disk to select spectral signals which enter an image collection module and an image display module through the optical filter disk to achieve the image collection of the tissue to be detected. Aiming at the different fluorescent probes, the corresponding suitable optical filters can enter the light passing positions only through turning the optical filter disk, and therefore, according to the system and method, the multispectral fluorescence imaging of various fluorescent probes can be achieved conveniently.

Description

technical field [0001] The invention relates to a multispectral fluorescence imaging system and imaging method based on a rigid endoscope. Background technique [0002] Traditional endoscopes can enter human tissues (animal tissues) for visual inspection in vivo, which improves the diagnosis level of tumor tissues in vivo. However, due to the limitation of technical indicators such as resolution, it is still not possible to detect small lesions that cannot be recognized by the naked eye. Do the screening. At present, endoscopes with fluorescence diagnostic functions have appeared in the world. Laser fluorescence diagnosis uses laser light to excite the affinity fluorescent agent in tumor cells, which produces a fluorescence effect different from that of surrounding normal tissues, and can clearly show the shape of tumor tissue. And location, so that doctors can easily distinguish and diagnose tiny tumor tissues invisible to the naked eye. [0003] However, most of the exis...

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Problems solved by technology

[0014] The purpose of the present invention is to provide a multispectral fluorescence imaging system based on a rigid endoscope to solve the problem that the rigid endoscope in the prior art can only excite and image specific fluorescent probes; the purpose of the present invention is also to A method for performing fluorescence imaging using the above-mentioned fluorescence imaging system is provided

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