Tissue culture and propagation method of cinnamomum camphora stem segments

Abstract

The invention relates to a tissue culture and propagation method of cinnamomum camphora. According to the method, the combination of improved DCR culture medium and MS culture is used for the first time; through the measures of improving the DCR culture medium element content, regulating the plant hormone level, increasing active carbon, regulating and controlling the temperature illumination, increasing the seeding strengthening culture and the like, the invention provides a cinnamomum camphora tissue culture system with the advantages that the bud induction rate reaches up to 98 percent or higher; the growth coefficient with an average of 4.8; the browning rate is smaller than 3.2 percent; the seeding strengthening rate (3cm or higher) is 81 percent or higher; the transplanting survival rate reaches 87 percent or higher; the heavy demand of markets on the cinnamomum camphora is met.

Description

technical field [0001] The invention relates to a method for tissue culture and propagation of Cinnamomum camphora, belonging to the technical field of tree tissue culture and propagation. Background technique [0002] Cinnamomum camphora (L.) Presl is a representative tree species of subtropical evergreen broad-leaved forest in eastern Asia. It is mainly distributed in Taiwan Province of my country and several provinces south of the Yangtze River. It is a precious multi-purpose local economic tree species in southern my country. National second-level key protected wild plants. Cinnamomum camphora has rounded branches, a large crown with thick shade, and rich leaf color. It not only beautifies the environment, but also has the functions of greening and scenting the environment. It has many ecological functions such as sound insulation, insect repellent, and air purification. It has been a southern village since ancient times. The best Fengshui forest and landscape tree speci...

AI Technical Summary

Problems solved by technology

However, there are disadvantages such as strong seasonality and difficulty in retaining good traits in seedlings

The second is to raise seedlings by cuttings, but it needs more ear picking mother plants. At the same time, the cuttings are greatly affected by environmental conditions, and the rooting effect is difficult to stabilize.

However, due to the characteristics of camphor plants, there are still difficulties in the conventional tissue culture process, such as low bud induction rate, easy browning of stem segments in subculture propagation, thin and weak tissue culture seedlings, and low transplant survival rate. An important reason why it is difficult to popularize and apply the industrialization of camphor

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