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Technology for preparing rare-earth tailings amelioration inocula by culturing plant growth-promoting bacteria with piggery wastewater as raw material

A technology of plant growth-promoting bacteria and plant growth-promoting bacteria, which is applied in the direction of bacteria, fertilizer mixtures, and methods based on microorganisms, which can solve the problems of high pollutant concentration and achieve the effects of increasing soil fertility, low price, and wide sources

Active Publication Date: 2017-07-14
JIANGNAN UNIV
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

This process is more convenient and fast, but at the same time, pig manure, feed residue and pig excrement will be washed into the wastewater together, so the wastewater contains a large amount of suspended solids, etc., and the concentration of other pollutants is also high

Method used

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Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0021] 1) Bacillus amyloliquefaciens H47 with preservation number CCTCC M 2013283, commercially available Bacillus licheniformis, commercially available Bacillus pumilus and other strains are activated and prepared into plant growth-promoting bacteria:

[0022] (1) Take a ring on the slope of the three bacterial strains, draw a line on the PDA plate to inoculate the hyphae, and cultivate in an incubator at 30°C for 18 hours;

[0023] (2) On the PDA plate, single colonies of three kinds of bacteria were picked and inserted into 250ml Erlenmeyer flasks equipped with 50ml LB liquid culture solution respectively, 12 of each bacteria were inoculated, cultured on a shaking table, culture conditions: rotating speed 180r / min, The temperature is 28° C., and the constant temperature is cultivated for 36 hours to obtain the seeds of the expanded cultivation of the plant growth-promoting bacterium agent.

[0024] 2) Pretreatment of pig farm wastewater: Weigh 3 kg of pig farm wastewater, a...

Embodiment 2

[0027] 1) Bacillus amyloliquefaciens H47 with preservation number CCTCC M 2013283, commercially available Bacillus licheniformis, commercially available Bacillus pumilus and other strains are activated and prepared into plant growth-promoting bacteria:

[0028] (1) Take one ring respectively on the slope of the three strains, draw a line on the PDA plate to inoculate the hyphae, and cultivate in an incubator at 30°C for 20 hours;

[0029] (2) On the PDA flat plate, pick three kinds of bacteria single colonies and insert them into 250ml Erlenmeyer flasks equipped with 50ml LB liquid culture solution respectively, inoculate 12 of each bacteria, shake table culture, culture conditions: rotating speed 200r / min, The temperature is 26° C., and the constant temperature is cultivated for 32 hours, and the seeds of the expanded cultivation of the plant growth-promoting bacteria agent are obtained.

[0030] 2) Pretreatment of pig farm wastewater: Weigh 3 kg of pig farm wastewater, adjus...

Embodiment 3

[0033] 1) Bacillus amyloliquefaciens H47 with preservation number CCTCC M 2013283, commercially available Bacillus licheniformis, commercially available Bacillus pumilus and other strains are activated and prepared into plant growth-promoting bacteria:

[0034] (1) Take a ring respectively on the slope of the three bacterial strains, draw a line on the PDA plate to inoculate the hyphae, and cultivate in an incubator at 30°C for 22 hours;

[0035] (2) On the PDA flat plate, pick three kinds of bacteria single colonies and insert them into 250ml Erlenmeyer flasks equipped with 50ml LB liquid culture solution respectively, inoculate 12 of each bacteria, shake table culture, culture conditions: rotating speed 160r / min, The temperature is 32° C., and the constant temperature is cultivated for 28 hours to obtain the seeds of the expanded cultivation of the plant growth-promoting bacterium agent.

[0036] 2) Pretreatment of pig farm wastewater: Weigh 3 kg of pig farm wastewater, adju...

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Abstract

The invention provides a technology for preparing rare-earth tailings amelioration inocula by culturing plant growth-promoting bacteria with piggery wastewater as a raw material. The technology is characterized in that the agricultural waste piggery wastewater is used as a raw material for culturing plant growth-promoting bacteria; and bacillus amyloliquefaciens H47 collected in the China Center for Type Culture Collection on June 24, 2013 with the collection No. of CCTCC M 2013283, commercial bacillus licheniformis and commercial Bacillus pumilus are used as the plant growth-promoting bacteria for preparation of the rare-earth tailings amelioration inocula. The piggery wastewater is used as the raw material for culturing the plant growth-promoting bacteria for preparation of the rare-earth tailings amelioration inocula. By the utilization of the plant growth-promoting bacteria for ammonium decomposition, soil fertility is increased. The product also can be used as a water-retaining agent, thus promoting formation of a soil aggregate structure and preventing soil hardening, destroying soil capillarity and avoiding soil moisture evaporation. By using the piggery wastewater as the raw material for culturing the plant growth-promoting bacteria, the sources are wide and price is low, and bacterial strain propagation speed is fast. In addition, the product also can be used as a fertilizer, providing various nutrients for plants. The technology is simple, has low production cost, and is easy for promotion. By the technology, piggery wastewater is reasonably utilized, thus changing waste into valuable things.

Description

technical field [0001] The invention relates to a technology for repairing ionic rare earth tailings, in particular to a technology for cultivating plant growth-promoting bacteria with agricultural waste pig farm wastewater as raw materials to prepare rare earth tailings improving bacterial agents. Belongs to the field of biotechnology. Background technique [0002] Heavy metal pollution in soil is mostly caused by human activities. Among them, the mining and smelting of heavy metals are more harmful to the surrounding soil environment. The tailings produced during the mining and smelting of heavy metals, due to untimely treatment, long-term accumulation and rainwater immersion, lead to the migration of heavy metals to the surrounding soil, which can cause very serious heavy metal pollution to the ecological environment in which they are located. Due to the large area of ​​contaminated soil, traditional physical and chemical remediation methods are difficult to implement d...

Claims

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Application Information

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Patent Type & Authority Applications(China)
IPC IPC(8): C12N1/20C09K17/14C05F11/08C12R1/10C12R1/07
CPCC05F11/08C09K17/14C12N1/20Y02P20/141
Inventor 廖祥儒田乔鹏窦欣蔡宇杰管政兵
Owner JIANGNAN UNIV
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