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Application of Phloretin in Preparation of Medicine for Treating Chicken Necrotic Enteritis

A technique for chicken necrotizing enteritis and necrotizing enteritis, which is applied in the field of medicine

Active Publication Date: 2019-11-19
BAODING JIZHONG PHARMA
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

However, as of now, there is no relevant research on the use of phloretin in the treatment of chicken necrotic enteritis caused by Clostridium perfringens at home and abroad.

Method used

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  • Application of Phloretin in Preparation of Medicine for Treating Chicken Necrotic Enteritis
  • Application of Phloretin in Preparation of Medicine for Treating Chicken Necrotic Enteritis
  • Application of Phloretin in Preparation of Medicine for Treating Chicken Necrotic Enteritis

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Experimental program
Comparison scheme
Effect test

Embodiment Construction

[0011] sliding motion inhibition test

[0012] The standard strain of Clostridium perfringens ATCC13124 was anaerobically cultured in liquid BHI medium at 37°C for 12 hours, and then 300 μl of the culture was taken to continue culture in liquid BHI, and cultured anaerobically at 37°C for 5 hours. Take 1ml of the culture, centrifuge at 10,000g to discard the supernatant, resuspend the bacteria in 500μl of BHI liquid medium, and make it double concentrated. Afterwards, 5 μl of the concentrated bacterial solution was added dropwise to 0.7% BHIA plates containing 4 μg / ml and 16 μg / ml phloretin, which were prepared in advance and dried in an oven at 37° C. for 1 hour, cultured anaerobically at 37° C. for 48 hours, and images were collected ( attached figure 2 ). The results showed that both 4μg / ml and 16μg / ml phloretin could significantly inhibit the sliding movement of Clostridium perfringens on BHIA, and the effect of 16μg / ml phloretin was more obvious.

[0013] Biofilm forma...

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Abstract

The invention relates to application of phloretin in preparation of a medicine for treating chicken necrotic enteritis. Through a clostridium perfringens sliding motion inhibition test, a biofilm formation inhibition test and a chicken necrotic enteritis model test, it is verified that phloretin has a protective effect on chicken necrotic enteritis caused by clostridium perfringens infection. In view of abuse of traditional antibiotics and continuous strengthening of bacterial resistance, phloretin has characteristics of high cure rate and no drug resistance or drug residue. Thus, phloretin can be used in treating chicken necrotic enteritis to increase selectivity of drug use and is of great significance for development of new drugs.

Description

technical field [0001] The invention relates to the application of phloretin in the preparation of medicine for treating chicken necrotic enteritis, which belongs to the field of medicine. Background technique [0002] Clostridium perfringens is an anaerobic Gram-positive bacillus, which widely exists in soil, water, food, human and animal feces, livestock feed and animal intestines in nature, and is the normal intestinal flora of humans and animals , an opportunistic pathogen. At present, according to the different exotoxins it secretes, it is divided into 5 serotypes, A-E, and poultry are mainly infected with type A and type C Clostridium perfringens, especially type A. After Clostridium perfringens infects poultry, it mainly causes necrotic enteritis, and its symptoms are manifested as listlessness, loose feathers, loss of appetite or waste, hematochezia, and severe decline in production performance. The lesions are mainly concentrated in the small intestine, showing th...

Claims

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Application Information

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Patent Type & Authority Patents(China)
IPC IPC(8): A61K31/12A61P1/00A61P31/04
CPCA61K31/12
Inventor 邓旭明刘水王建锋周永林王婷婷
Owner BAODING JIZHONG PHARMA
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