A method for removing sterols in herbivorous insect artificial feed

Abstract

The invention provides a method of removing sterols in artificial feed for phytophagous insects. The method comprises the steps of: 1) adding anhydrous ethanol to a container filled with plant seed powder, stirring the mixture and allowing the mixture to stand, and sucking a supernatant away; 2) adding n-hexane, stirring the mixture and allowing the mixture to stand, and sucking a supernatant away; 3) evaporative-drying the supernatants in the steps 1) and 2), and performing elution separation in a column chromatography manner; 4) separating the liquid, after elution in the step 3) into two samples, one with the sterols, the other without the sterols; 5) through thin-layer chromatography, separating the sample with the sterols in the step 4), and respectively scraping zones with and without the sterols; 6) extracting the sterols in the step 5), and performing elution separation in a column chromatography manner; and 7) mixing the substances without the sterols in the steps 1) to 6), and removing the solvent to prepare a sterol-free feed raw material. The method is low in technical requirement, is easy to carry out, is short in removal period and is free of loss of other nutrients except the sterols.

Description

technical field [0001] The invention relates to the field of insect artificial feed, in particular to a method for removing sterols from herbivorous insect artificial feed. Background technique [0002] Sterols are the general term for four-membered ring lipids, most of which are similar in structure. The basic skeleton is cyclopentane polyhydrophenanthrene nucleus, which consists of three six-membered rings and one five-membered ring. The difference between different sterols lies in the C-3 position The functional groups connected, the side chains connected to the C-17 position, and the position of the double bond on the side chain are as follows: [0003] [0004] Sterols include animal sterols (cholesterol) and phytosterols. At present, more than 250 sterols have been identified, and they are mainly in the free state, sterol esters (fatty acid esters and phenolic esters), steryl glycosides and acylated steryl glycosides, etc. form exists. Common free sterols include ...

AI Technical Summary

Problems solved by technology

Because these methods of removing sterols are only to extract sterols and a part of non-sterol nutrients together with extractants extensively, without further separation, which will cause changes in other nutrients in the raw materials

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