Asexual propagation method of hizikia fusiforme

A technology of asexual reproduction and hijiki, applied in horticultural methods, botanical equipment and methods, climate change adaptation, etc., can solve the problems that limit the development of artificial breeding and cannot be completely solved, and achieve simple and easy operation and induction short cycle effect

Inactive Publication Date: 2017-07-04
QINGDAO QINGQUAN BIOTECH CO LTD
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

[0003] Due to the supply of seedlings, it is still not completely resolved, thus limiting the development of artificial breeding
At present, there are few reports on tissue culture research of Hijiki

Method used

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Examples

Experimental program
Comparison scheme
Effect test

Embodiment Construction

[0010] The present invention will be described in further detail below in conjunction with specific embodiments.

[0011] A method for asexual propagation of Hijiki, the steps are as follows:

[0012] (1) Choose healthy immature sargassum, wash it several times, and then rinse it with sterile seawater to remove the parasitic protozoa and epiphytic algae on the surface of the algae. After culturing with sterile seawater for a week, wash it repeatedly until microscopic examination Soak in 1.5% KI solution for 10 minutes until there are no algae on the surface of the algae, then rinse 3 times with sterilized seawater, soak in 70% alcohol for 10 seconds, and then rinse 3 times with sterilized seawater;

[0013] (2) Cut the treated Sargassum algae into 2-4 mm sections with a scalpel, culture them in PES medium containing 0.25 mg / mL 6-BA, and culture them in the dark for 7 days;

[0014] (3) Change the culture conditions to: temperature 17°C, light intensity 10±2 µE•m −2 •s −1 , ...

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Abstract

The invention relates to an asexual propagation method of hizikia fusiforme. The method comprises the following steps that (1) healthy unripe hizikia fusiforme is selected, and washed for multiple times and then the washed hizikia fusiforme is flushed with disinfectant seawater to remove parasitic protozoa and adnascent hybrid seaweeds on the surfaces of fronds; after being cultured with the disinfectant seawater for one week, the healthy unripe hizikia fusiforme is washed repeatedly until no hybrid seaweeds exist on the surface of the fronds through microscopic examination; and the washed-repeatedly hizikia fusiforme is soaked in a solution KI of 1.5% for 10min, the soaked hizikia fusiforme is washed with the disinfectant seawater for three times, the washed hizikia fusiforme is soaked with alcohol of 70% for 10s, and then the soaked hizikia fusiforme is washed with the disinfectant seawater for three times; (2) the treated hizikia fusiforme fronds are cut into segments of 2-4mm by using a surgical blade, the hizikia fusiforme segments are cultured in a PES culture solution containing 0.25mg / mL of 6-BA in darkness for 7 days; and (3) the culture conditions are changed as follows: the temperature is 17 DEG C, the illumination intensity is 10+ / -2[mu]E.m<-2>.s<-1>, and the photoperiod is 16h:8h (Light:Dark). The asexual propagation method is easy to operate and implement and is short induction duration, provides a technical method for artificial breeding and seedling culture of the hizikia fusiforme and also provides a support for production of hybrid seeds and propagation expansion.

Description

technical field [0001] The invention relates to a method for asexual reproduction of hijiki. Background technique [0002] Hizikia fusifarme (Hizikia fusifarme), an algae plant, is also known as antler tip, sea vegetable bud, goat milk, sea barley, etc. Yuanzigang, Sargassumaceae. The algal body is yellow-brown, plump and juicy, and the fronds vary greatly and have various shapes. Plant height 30-50cm, up to 3m or more. Divided into rhizomes, trunks, leaves and air sacs. Rhizoid terete with branches. The primary leaves below the "stem" are flat and thick, oval, and the epigenetic "leaves" on the upper part of the "stem" are rod-shaped or eggplant-shaped. Air sacs spindle-shaped, born in leaf axils. Dioecious plants, oocysts and sperm sacs are formed in the reproductive receptacle. It grows on rocks in the low tide zone, and grows luxuriantly along the coast of southern China, and is also distributed in Shandong, Liaoning and other places. After the 1990s, large-scale...

Claims

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Application Information

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Patent Type & Authority Applications(China)
IPC IPC(8): A01G33/00A01H4/00
CPCA01G33/00A01H4/008Y02A40/80
Inventor 褚方强
Owner QINGDAO QINGQUAN BIOTECH CO LTD
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