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Method for optimizing shake-flask fermentation process for producing high-yield lipopeptide antibiotics by bacillus subtilis T-500

A technology of Bacillus subtilis and lipopeptide antibiotics, applied in the field of shake flask fermentation process optimization, can solve the problem of low content of lipopeptide antibiotics, achieve the effect of increasing the content of fermentation bacteria and increasing the bandwidth of antibacterial

Active Publication Date: 2017-06-23
JIANGSU ACADEMY OF AGRICULTURAL SCIENCES
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Problems solved by technology

[0005] The purpose of the present invention is to solve the low content of lipopeptide antibiotics in the existing Bacillus subtilis T-500 dry suspension powder, and provide a kind of shaking flask fermentation process to Bacillus subtilis T-500 high-yield lipopeptide antibiotics An optimized method, the present invention provides technical support and theoretical guidance for the industrial production of Bacillus subtilis T-500 strain and the acquisition of high-yield lipopeptide antibacterial substances

Method used

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  • Method for optimizing shake-flask fermentation process for producing high-yield lipopeptide antibiotics by bacillus subtilis T-500
  • Method for optimizing shake-flask fermentation process for producing high-yield lipopeptide antibiotics by bacillus subtilis T-500
  • Method for optimizing shake-flask fermentation process for producing high-yield lipopeptide antibiotics by bacillus subtilis T-500

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Embodiment

[0031] Bacillus subtilis (Bacillus subtilis) T-500 and rice sheath blight (Rhizoctoniasolani) RH-2 were preserved by the Rice Disease and Biological Control Laboratory, Institute of Plant Protection, Jiangsu Academy of Agricultural Sciences.

[0032] The routine activation of Bacillus subtilis T-500 uses LB medium (tryptone 10.0g, yeast powder 5.0g, NaCl 10.0g), and the common medium YPG (tryptone 5.0g / L, yeast extract 5.0g / L) for fermentation seed liquid , glucose 5.0g / L) preparation. Rice sheath blight RH-2 was activated and cultured with PDA medium (potato 200.0g / L, glucose 20.0g / L, agar 15.0g / L). The 10 basic fermentation media commonly used in this study are listed in Table 1.

[0033] Table 1 Type and composition of culture medium

[0034]

[0035]

[0036] Preparation of fermented seed liquid: Pick T-500 strains stored in a refrigerator at -70°C and streak them onto LB solid plates, and after standing at 28°C for 24 hours, pick single cells and inoculate them in...

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Abstract

The invention particularly relates to a method for optimizing a shake-flask fermentation process for producing high-yield lipopeptide antibiotics by bacillus subtilis T-500, and belongs to the technical field of bacillus subtilis T-500 lipopeptide antibiotic fermentation processes. According to the method, rhizoctonia solani is adopted as an indicator, the lipopeptide antibiotics are extracted on the basis of 10 common fermentation culture media by virtue of an acid precipitation method, the bacteriostatic effect of a T-500 crude extract is analyzed, and fermentation culture medium components with influence on the bacteriostatic effect are screened; fermentation culture medium components and fermentation conditions for production of the high-yield lipopeptide antibiotics by the T-500 are optimized through a Plackett-Burman experimental design, a CCD (central composite design) and a response surface method. According to the method, a technical support and a theoretical guide are provided for industrially producing and obtaining high-yield lipopeptide antibiotic substances by a bacillus subtilis T-500 strain.

Description

technical field [0001] The invention belongs to the technical field of fermentation technology of bacillus subtilis T-500 lipopeptide antibiotics, and in particular relates to a method for optimizing the shake flask fermentation technology of bacillus subtilis T-500 high-yield lipopeptide antibiotics. Background technique [0002] Bacillus is a kind of bio-control bacteria resource that has been researched deeply at present. It has a developed secretion system and strong environmental adaptability, and is antagonistic to various plant pathogenic bacteria. Pathogens develop drug resistance, which has been widely used in the creation and development of biopesticides. The active strains that have been promoted and applied at home and abroad include Bacillus amyloliquefaciens FZB42, Bacillus subtilis GB03 and Bacillus pumilus GB34, etc.; the microbial fungicide "Venquning" developed by Chen Zhiyi and others, its active ingredient is Bacillus subtilis Bs916, the preparation is ef...

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Application Information

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IPC IPC(8): C12P21/00C12N1/20C07K14/32C07K1/30C12R1/125
CPCC07K14/32C12N1/20
Inventor 乔俊卿刘永锋刘邮洲张荣胜
Owner JIANGSU ACADEMY OF AGRICULTURAL SCIENCES
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