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Use of chicken anemia virus (CAV) VP1-aa 1-19 polypeptide as highly efficient cell-penetrating peptide

A technology for chicken infectious anemia and membrane-penetrating peptides, which is applied in the field of high-efficiency cell-penetrating peptides and can solve problems such as weakening the membrane-penetrating ability.

Active Publication Date: 2017-06-13
YANGZHOU UNIV
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  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

However, the TAT short peptide contains two Furin enzyme recognition cleavage sites (ie RKKR and RQRR), so TAT will be hydrolyzed by Furin during the membrane penetration process to destroy the TAT structure, thereby weakening its membrane penetration ability

Method used

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  • Use of chicken anemia virus (CAV) VP1-aa 1-19 polypeptide as highly efficient cell-penetrating peptide
  • Use of chicken anemia virus (CAV) VP1-aa 1-19 polypeptide as highly efficient cell-penetrating peptide
  • Use of chicken anemia virus (CAV) VP1-aa 1-19 polypeptide as highly efficient cell-penetrating peptide

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Embodiment Construction

[0026] In order to better understand the content of the present invention, the following embodiments illustrate the preparation and identification process of a high-efficiency cell-penetrating peptide derived from the chicken infectious anemia virus VP1-aa 1-19 polypeptide in conjunction with the accompanying drawings.

[0027] Implementation process

[0028] (1) Preparation and synthesis of chicken infectious anemia virus VP1-aa 1-19 polypeptide

[0029] Using the online database UniProt (http: / / www.uniprot.org / uniprot / ), the N-terminus (aa 1-60) of the VP1 sequence from different CAV reference strains was compared, analyzed and marked in the sequence Positively charged polar amino acids (such as figure 1 ). According to the annotation information of the Family&Domains module, the N-terminal (aa1-60) sequence of the CAV VP1 sequence was characterized. It was found that the N-terminus of VP1 was highly conserved. According to the UniProt database information annotation, th...

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Abstract

The invention relates to a use of a chicken anemia virus (CAV) VP1-aa 1-19 polypeptide as a highly efficient cell-penetrating peptide. The VP1-aa 1-19 polypeptide sequence is shown in SEQ ID NO. 2. The polypeptide can carry FITC and enter 293T cells, HCT-116 cells, 3T3 cells, MDCK cells and MSB1 cells in 30min, and the cell-penetrating efficiency is positively correlated with the concentration of the cell-penetrating peptide. The VP1-aa 1-19 polypeptide can efficiently enter suspension culture cells. The laser confocal microscope and flow cytometry result shows that the CAV VP1-aa 1-19 polypeptide has cell-penetrating efficiency significantly higher than that of a TAT short peptide.

Description

technical field [0001] The invention relates to a high-efficiency cell-penetrating peptide derived from chicken anemia virus (Chicken anemia virus, CAV) VP1-aa 1-19 polypeptide. The polypeptide can carry FITC and penetrate into different cells (including adherent cells and suspension cells) within 30 minutes. Compared with TAT, it was found that the polypeptide has a significantly higher cell-penetrating function on FITC than TAT. Therefore, the high-efficiency cell-penetrating peptide derived from chicken infectious anemia virus VP1-aa 1-19 polypeptide obtained in the present invention has certain application prospects and value. Background technique [0002] Cell penetrating peptides (CPPs) are short peptides that can carry biomacromolecules into cells, generally 5-30 in length and rich in positively charged basic amino acids. It can not only efficiently pass through the cell membrane and enter cells, but also efficiently carry different types of exogenous molecules such...

Claims

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Application Information

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IPC IPC(8): C07K14/01
CPCC07K14/005C12N2750/10022
Inventor 叶建强呼高伟邵红霞秦爱建刘敏申秋平田晓彦金甫
Owner YANGZHOU UNIV
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