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Detecting typing kit for susceptibility gene of esophagus cancer and application thereof

A susceptibility gene and kit technology, applied in the field of testing, can solve problems such as limiting, affecting the biological activity of TGF-β signaling pathway, and loss of tumor suppressor function, so as to achieve the effect of predicting disease risk.

Inactive Publication Date: 2017-06-09
SHANGHAI YIRUN BIOLOGICAL TECH
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AI Technical Summary

Problems solved by technology

The loss of Runx expression will directly lead to the limitation of Smad protein function, and then affect the biological activity of TGF-β signaling pathway, leading to the occurrence and development of tumors (Miyazono K, Suzuki H, Imamura T. Regulation of TGF-beta signaling and its roles in progression of tumors.[J].Cancer Science,2003,94(3):230-4.)
Studies have shown that the methylation of Runx1 gene is another major factor for its inactivation and low transcription (Reed-Inderbitzin E, Moreno-Miralles I, Vanden-Eynden S K, et al. RUNX1 ass ociates with histone deacetylases and SUV39H1 to represstranscriptio n.[J ].Oncogene,2006,25(42):5777-86.), leading to the loss of its tumor suppressor function

Method used

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  • Detecting typing kit for susceptibility gene of esophagus cancer and application thereof
  • Detecting typing kit for susceptibility gene of esophagus cancer and application thereof
  • Detecting typing kit for susceptibility gene of esophagus cancer and application thereof

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Embodiment Construction

[0026] The technical solution of this patent will be further described in detail below in conjunction with specific embodiments.

[0027] An esophageal cancer susceptibility gene detection and typing kit, the kit includes a DNA extraction kit, a DNA amplification kit and a sequencing kit; the DNA extraction kit adopts the DP322 type of Tiangen Biochemical Technology Co., Ltd. Product, the DNA amplification kit mainly includes amplification primers, enzymes, PCR Buffer, d NTP Mixture, amplification primers, the amplification primers are: 1) F: 5'-GGGGAATGAGACAGAGACCA-3' (SEQ ID NO .1), R: 5'-ACCATTCCCAATGTCTGCTC-3' (SEQ ID NO.2), 2) F: 5'-GGAAGCAGTGAGGTGCAGAGGT-3' (SEQ ID NO.3), R: 5'-TCCACAACTGCAAAACGAA GAAA-3' ( SEQ ID NO.4), 3) F: 5'-TCTCCAGCCCATGGTGAGCATTA-3' (SEQ ID NO.5), R: 5'-CCCCCATATGGTATCCCCCAAAG-3' (SEQ ID NO.6); Company's TaKaRa Taq TM enzyme; the sequencing kit adopts the Terminator v3.1Cycle Sequencing Kit.

[0028] The amplification primers are designed and...

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Abstract

The invention discloses a detecting typing kit for a susceptibility gene of esophagus cancer and an application thereof. The detecting typing kit for the susceptibility gene of esophagus cancer can detect three polymorphic loci PED4D, PLCE1 and RUNX1 of an esophagus cancer related gene, so that the genetic factors of an individual in the aspect of esophagus cancer can be known, the relative risk of esophagus cancer can be estimated, and the kit has positive meaning in diagnosing, early treating and intervening esophagus cancer. Meanwhile, the kit also can assist diagnosis of esophagus cancer, guide reasonable medication, and is helpful for predicating morbid risk of offsprings and the like.

Description

technical field [0001] The invention relates to the technical field of testing, in particular to a detection and typing kit for esophageal cancer susceptibility genes and its application. Background technique [0002] With the completion of the Human Genome Project (HGP), the discovery of a large number of disease-related genes has promoted the transformation of the traditional biomedical model into a predictable, preventable, individualized and participatory genomic medicine model, which will provide a better way for the future. The development of prevention, diagnosis, and treatment of major and complex diseases such as cancer, cardiovascular and cerebrovascular diseases, diabetes, neurological and mental diseases, which have long plagued human beings, has opened up new ways and also provided good opportunities for the industrialization of genetic science. [0003] Genome Wide Association Studies (GWAS) is a case-control association analysis using millions of single nucleo...

Claims

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Application Information

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IPC IPC(8): C12Q1/68
CPCC12Q1/6869C12Q1/6886C12Q2600/106C12Q2600/118C12Q2600/156C12Q2531/113C12Q2535/101
Inventor 何侃栗娟王佳其他发明人请求不公开姓名
Owner SHANGHAI YIRUN BIOLOGICAL TECH
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