Skeletal muscle tissue frozen section myosin adenosine triphosphatase dyeing kit
An adenosine triphosphate and frozen section technology, which is applied in biological testing, material inspection products, measuring devices, etc., can solve the problems of waste of reagents, poor dyeing effect, time-consuming and other problems, and achieve the effect of improving work efficiency, convenient effect and saving reagents.
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[0037] Myosin adenosin triphosphate (ATPase) staining kit for frozen sections of skeletal muscle tissue, the kit contains the following components:
[0038] (1) CaCl 2 Buffer solution (No. 1 solution): 0.1mol / L glycine solution, 0.1mol / L NaCl solution, 1 mol / LCaCl 2 , 0.1 mol / L NaOH, adjust the pH value to 9.5±0.01 with 0.1 mol / L NaOH;
[0039] (2) Acetate buffer solution (No. 2 solution): 0.2 mol / L sodium acetate, 0.2 mol / L acetic acid, adjust the pH value to 4.6±0.01 with 0.1 mol / L HCl;
[0040] (3) Substrate incubation solution (No. 3 solution): Take the CaCl in No. 1 solution 2 10ml of buffer solution, 25mg of ATP-disodium salt, adjust the pH to 9.4±0.01 with 0.1mol / L NaOH;
[0041] (4) 2wt.% cobalt chloride solution (No. 4 solution);
[0042] (5) Ammonium sulfide solution (No. 5 solution): Ammonium sulfide working solution: 1wt% ammonium sulfide solution, freshly prepared before use.
[0043] No. 1-3 liquids should be stored frozen at -20°C, -35°C or -80°C; liquids 4...
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