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Method for establishing and applying full-time multi-wavelength overlay fingerprint spectrum of rhizoma chuanxiong decoction pieces

A fingerprint and full-time technology, which is applied to the construction and application of the full-time multi-wavelength superimposed fingerprint of Chuanxiong decoction pieces, can solve the problem of difficult to reflect component information, and achieve the effect of a reliable evaluation method

Inactive Publication Date: 2017-05-31
绵阳市农业科学研究院
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  • Abstract
  • Description
  • Claims
  • Application Information

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Problems solved by technology

However, due to the complex and diverse chemical components of Chinese herbal medicines, it is difficult to reflect all component information in fingerprints under the current single-wavelength detection.

Method used

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  • Method for establishing and applying full-time multi-wavelength overlay fingerprint spectrum of rhizoma chuanxiong decoction pieces
  • Method for establishing and applying full-time multi-wavelength overlay fingerprint spectrum of rhizoma chuanxiong decoction pieces
  • Method for establishing and applying full-time multi-wavelength overlay fingerprint spectrum of rhizoma chuanxiong decoction pieces

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Embodiment Construction

[0021] According to the examples, the present invention will be further explained:

[0022] The construction and application method of the whole-time multi-wavelength superimposed fingerprint of the Chuanxiong decoction piece of the present invention will be further described in the following specific embodiments. The following examples include the precision, stability, and repeatability tests of the method. These embodiments are only exemplary and do not constitute any limitation to the scope of the present invention.

[0023] 1. Sample source and reagents

[0024] 16 chuanxiong decoction pieces were purchased from major domestic pharmacies. In order to verify the reliability and specificity of the fingerprints, two sources of Ligusticum chinensis and Angelica decoction pieces from two sources were randomly purchased at the same time as the outer group. All samples were dried at 40°C to constant weight before analysis, crushed through a 50-mesh sieve, and stored in a refrigerator ...

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Abstract

The invention provides a method for establishing and applying a full-time multi-wavelength overlay fingerprint spectrum of rhizoma chuanxiong decoction pieces. The method is characterized by sequentially comprising the following steps: (1) preparation of a test solution; (2) preparation of chromatographic conditions; (3) establishment of a full-time multi-wavelength overlay fingerprint spectrum; and (4) principal component analysis and clustering analysis. The method for establishing and applying the full-time multi-wavelength overlay fingerprint spectrum of rhizoma chuanxiong decoction pieces, provided by the invention, is combined with the modern test analysis technology and an information processing method to provide a comprehensive, stable and reliable assessment method for the quality control of a large amount of rhizoma chuanxiong as the traditional Chinese medicinal material.

Description

Technical field [0001] The invention belongs to the technical field of quality detection of Chinese medicinal materials and Chinese patent medicines, in particular to the construction and application of a full-time multi-wavelength superimposed fingerprint spectrum of Chuanxiong decoction pieces. Background technique [0002] The traditional Chinese medicine Ligusticum chuanxiong is the rhizome of the perennial herb Ligusticum chuanxiong (Ligusticum chuanxiongHort.) of the Umbelliferae genus Ligusticum. It is mainly produced in Sichuan Province and is also cultivated in Yunnan, Guizhou, Gansu and other provinces. Ligusticum chuanxiong has the effects of invigorating blood, promoting qi, dispelling wind and relieving pain, and is often used in the treatment of chest pain, chest pain, chest hypochondriac tingling, and irregular menstruation. Modern chemical and pharmacological studies have shown that the chemical components of Ligusticum chuanxiong mainly include volatile oil, phen...

Claims

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Application Information

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Patent Type & Authority Applications(China)
IPC IPC(8): G01N30/02
CPCG01N30/02
Inventor 王涛
Owner 绵阳市农业科学研究院
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