Preparation method and application of algal liquid for fluorescence detection of haze biotoxicity by Chlorella pyrenoidosa

Abstract

The preparation method of algae liquid for measuring the biological toxicity of chlorella pyrenoidum by fluorescence, belongs to the technical field of chlorella pyrenoidum, including the cultivation of algae species and the preparation of algae liquid, specifically including the following steps: A. Selecting pyrenoid globules Algae species, under sterile conditions, inoculate the algae species into the Erlenmeyer flask equipped with liquid medium, after the inoculation is completed, place it in a constant temperature light incubator and cultivate it to the logarithmic phase, and set aside; B, culture to the logarithmic phase After the algae liquid obtained in the stage is placed in the dark, measure the chlorophyll fluorescence kinetic parameters of the algae liquid with a water sample chlorophyll fluorescence meter; ×10 6 ind / ·ml ‑1 The algae liquid of Chlorella pyrenoidosa in the logarithmic phase can be used for haze biotoxicity determination. The invention also provides the measurement of the biotoxicity of the chlorella pyrenoidosa liquid in the haze. The method of the invention is simple and easy to operate, and can intuitively reflect the biotoxicity of the haze.

Description

technical field [0001] The invention belongs to the technical field of chlorella pyrenoidosa, relates to the preparation of a chlorella pyrenoidosa liquid, in particular to a preparation method and application of a chlorella pyrenoidosa liquid for measuring haze biological toxicity. The preparation method of the chlorella pyrenoidosa liquid of the present invention is simple and easy to operate, and the prepared chlorella pyrenoidosa liquid has high sensitivity and intuitive reflection when used for measuring haze biotoxicity. Background technique [0002] Smog is a mixture of fog and haze. When the humidity is high in the morning and evening, there are many components of fog. When the humidity is low during the day, haze dominates, and the relative humidity is between 80% and 90%. Among them, fog is a natural weather phenomenon. Water vapor in the air is dense. Although dust is used as condensation nuclei, it is generally non-toxic and harmless. The core substances of haze ...

AI Technical Summary

Problems solved by technology

[0004] The present invention provides a preparation method and application of Chlorella pyrenoidosa liquid for measuring the biological toxicity of smog in order to solve the inability to intuitively reflect the toxicity of smog on organisms in the prior art, which can fully understand the smog The toxic effect of haze, and can better understand the toxicological mechanism and pathway of pollutants, and can better reflect the phenomenon and information of haze exposure in the actual environment

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