Preparation method and application of alga solution of chlorella pyrenoidosa, for detecting biological toxicity of haze by fluorescent light

Abstract

The invention provides a preparation method of an alga solution for detecting biological toxicity of haze by fluorescent light and belongs to the technical field of the chlorella pyrenoidosa. The preparation method comprises culture of alga seeds and preparation of the alga solution and specifically comprises the following steps: A, selecting the alga seeds of the chlorella pyrenoidosa and inoculating the alga seeds into a conical beaker filled with a liquid culture medium under a sterile condition; after inoculating, putting the conical beaker into a constant-temperature light illumination culture box to an exponential phase for later use; B, after putting the alga solution, which is cultured to the exponential phase, in a dark place, determining chlorophyll fluorescence dynamics parameters of the alga solution by utilizing a water sample chlorophyll fluorometer; C, selecting the alga solution of the chlorella pyrenoidosa at the exponential phase, which has the cell density range of (1 to 1.2)*10<6>idn / .ml<-1>, according to the chlorophyll fluorescence dynamics parameters of the alga solution in the step B, so as to determine the biological toxicity of the haze. The invention further provides application of the alga solution of the chlorella pyrenoidosa to determination of the biological toxicity of the haze. The method provided by the invention is simple and easy to operate and can directly reflect the biological toxicity of the haze.

Description

technical field [0001] The invention belongs to the technical field of chlorella pyrenoidosa, relates to the preparation of a chlorella pyrenoidosa liquid, in particular to a preparation method and application of a chlorella pyrenoidosa liquid for measuring haze biological toxicity. The preparation method of the chlorella pyrenoidosa liquid of the present invention is simple and easy to operate, and the prepared chlorella pyrenoidosa liquid has high sensitivity and intuitive reflection when used for measuring haze biotoxicity. Background technique [0002] Smog is a mixture of fog and haze. When the humidity is high in the morning and evening, there are many components of fog. When the humidity is low during the day, haze dominates, and the relative humidity is between 80% and 90%. Among them, fog is a natural weather phenomenon. Water vapor in the air is dense. Although dust is used as condensation nuclei, it is generally non-toxic and harmless. The core substances of haze ...

AI Technical Summary

Problems solved by technology

[0004] The present invention provides a preparation method and application of Chlorella pyrenoidosa liquid for measuring the biological toxicity of smog in order to solve the inability to intuitively reflect the toxicity of smog on organisms in the prior art, which can fully understand the smog The toxic effect of haze, and can better understand the toxicological mechanism and pathway of pollutants, and can better reflect the phenomenon and information of haze exposure in the actual environment

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