Device and method for fully automatically and continuously capturing and collecting single cells

Abstract

The invention discloses a device and a method for fully automatically and continuously capturing and collecting single cells. The device comprises a glass substrate, a PDMS (polydimethylsiloxane) microfluidics chip, an electromagnet, a permanent magnet matching part, a switch relay, a signal amplifying circuit and a signal collection control system, wherein the PDMS microfluidics chip is concavely engraved with a micro-channel; the micro-channel comprises a vertical main channel, a plurality of cell capturing channels and cell capturing holes; a sample inlet hole is formed in one end of the vertical main channel, the cell capturing channels are uniformly distributed at both sides of the vertical main channel, and each cell capturing hole is formed between the corresponding cell capturing channel and the main channel, and is provided with a cell detection gated channel; a cell collecting hole is formed in the tail end of each cell capturing channel; the electromagnet and the permanent magnet matching part are respectively positioned under the glass substrate and above the PDMS chip at a detection door. The device has the characteristics that the full automatic operation is realized; the single cells can be automatically captured and collected at high flux through the matching of the electromagnet, the permanent magnet matching part and the microfluidics chip.

Description

technical field [0001] The invention relates to the technical field of cell manipulation, in particular to a device and method for fully automatic and continuous capture and collection of single cells based on a microfluidic chip. Background technique [0002] Cell analysis is a core method in the field of biological and medical analysis. At present, the method of manual operation of the microscope is commonly used in related fields, which can ensure the accuracy, researchability and comprehensiveness of the analysis results. However, due to the process Time-consuming, low efficiency, large sample consumption, high cost, and high requirements for the professional level of operators are restricted in high-intensity and large-scale applications. [0003] The microfluidic chip device can integrate the basic operations of sample preparation, reaction, separation and detection in the process of biological, chemical and medical analysis into a micron-scale chip, and automatically ...

AI Technical Summary

Problems solved by technology

The advantages are simple operation, simple equipment, low cost, etc.; the disadvantages are weak manipulation and immature technology

[0010] In summary, there is currently no device and method with simple equipment and easy operation that can realize the continuous automatic capture and collection of single cells to meet the needs in the fields of biological research and medical analysis.

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