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Molecular marker for clinical prognosis of pancreatic cancer and application thereof

A technology for molecular markers and pancreatic cancer, applied in the determination/testing of microorganisms, biochemical equipment and methods, DNA/RNA fragments, etc., can solve the problems of genes not transcribing and translating, proteins losing biological functions, etc.

Active Publication Date: 2017-05-31
北京致成生物医学科技有限公司
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

These changes make such genes unable to transcribe and translate, or produce defective proteins and lose their original biological functions

Method used

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  • Molecular marker for clinical prognosis of pancreatic cancer and application thereof
  • Molecular marker for clinical prognosis of pancreatic cancer and application thereof

Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0041] Example 1 Survival analysis of pancreatic cancer based on TCGA database information Screening related genes

[0042] 1. Screening of clinical information

[0043] The clinical information of pancreatic cancer patients in the TCGA database was retrieved. As of December 10, 2015, a total of 185 clinical cases of pancreatic cancer were recorded in the TCGA database. These data were screened, and a total of 160 patients were included in the study. Patients with a history of other malignant tumors, who had received radiotherapy or chemotherapy were excluded during screening, and patients included in the study were required to contain clinical information and mRNA data.

[0044] 2. Survival study sample statistics

[0045] The statistical results of the survival time of 160 patients with pancreatic cancer are shown in the following table:

[0046] Table 1 Survival time statistics of 160 patients with pancreatic cancer

[0047] Survival time t (years) The numbe...

Embodiment 2

[0056] Example 2 RT-PCR detection of gene expression in pancreatic cancer and paracancerous tissues

[0057] 1. Materials

[0058] 1.1 Histopathological specimens

[0059] Tissues from 60 patients with pancreatic cancer who had been surgically resected and confirmed by pathological examination and corresponding paracancerous tissue samples were collected. Paracancerous tissue was defined as pancreatic tissue beyond 3 cm from the tumor edge. The samples were collected from patients who were diagnosed with pancreatic cancer and underwent surgical resection from October 2012 to December 2015 at Peking Union Medical College Hospital. Immediately after surgical resection of pancreatic cancer tissue, under the guidance of pathologists, pancreatic cancer and paracancerous tissues were taken and placed in liquid nitrogen, and stored in a -80°C low-temperature refrigerator after serial numbering.

[0060] 1.2 The inclusion criteria are as follows:

[0061] (1) Cases undergoing surgi...

Embodiment 3

[0099] The preparation of embodiment 3 kits

[0100] Based on the primer set obtained in Example 2, the kit for the prognostic diagnosis of pancreatic cancer according to the present invention was assembled, and the kit included a primer set for specific amplification of one or more genes in LOC127841, LOC402377, GEMIN8P4 and SLC6A10P as shown in the table 3, specifically:

[0101] 1. Kit 1 includes a pair of primers for specifically amplifying LOC127841;

[0102] 2. Kit 2 includes primer pairs for specific amplification of LOC127841, LOC402377, GEMIN8P4 and SLC6A10P.

[0103] The primer pair for specifically amplifying the housekeeping gene (GAPDH) is shown in SEQ ID NO: 9 and SEQ ID NO: 10; it also includes a SYBR Green polymerase chain reaction system, such as PCR buffer, SYBR Green fluorescent dye, and dNTPs. The composition of described PCR buffer is 25mM KCl, 2.5mM MgCl 2 , 200mM (NH 4 ) 2 SO 4 ; Also includes normal standard: used as a negative control and detecti...

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Abstract

The invention discloses a molecular marker for clinical prognosis of pancreatic cancer. The molecular marker is one or more genes selected from LOC127841, LOC402377, GEMIN8P4 and SLC6A10P. The invention further discloses application of the molecular marker to preparation of a pancreatic cancer clinical prognosis reagent or a kit. The reagent or kit comprises one or more groups of primer sequences from specific amplification LOC127841, LOC402377, GEMIN8P4 and SLC6A10P. By using the one or more genes as the marker to detect pancreatic cancer, precision is improved greatly, early detection and prognosis evaluation can be achieved quickly and effectively, and the molecular marker is of great significance for diagnosis and treatment of pancreatic cancer.

Description

technical field [0001] The invention relates to the field of biological detection, in particular to a molecular marker for clinical prognosis diagnosis of pancreatic cancer and its application. Background technique [0002] Pancreatic cancer is a common tumor of the digestive tract and one of the malignant tumors with the worst prognosis. According to the latest epidemiological survey, pancreatic cancer ranks fourth in the mortality rate of malignant tumors in developed countries such as Europe and the United States, and about 2.5 million people die of pancreatic cancer every year in the world. In the past 20 years, the incidence of pancreatic cancer in my country has continued to increase, and pancreatic cancer currently ranks fifth in the mortality rate of malignant tumors. In the treatment of pancreatic cancer, early diagnosis is difficult, its onset is hidden, it lacks typical clinical symptoms, and its prognosis is extremely poor. Pancreatic cancer is highly aggressive...

Claims

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Application Information

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IPC IPC(8): C12Q1/68C12N15/11
CPCC12Q1/6886C12Q2600/118C12Q2600/158C12Q2600/178
Inventor 刘昊
Owner 北京致成生物医学科技有限公司
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