Obtaining method and application of microbial flora for fermenting enteromorpha to prepare marsh gas

Abstract

The invention discloses an obtaining method and application of microbial flora for fermenting enteromorpha to prepare marsh gas. According to the obtaining method, the enteromorpha is used as a matrix; silt and seawater collected on an intertidal zone are utilized; the microbial flora is obtained through continuous fermentation and domestication. The flora obtained by the obtaining method can maintain favorable activity and a higher gas yield in the sweater of which the salinity is 1 percent to 2 percent, and degrades the enteromorpha in a system of which the salinity is 3.5 percent or below to generate the marsh gas; in the condition that an enteromorpha initial material is not washed by using fresh water, the high-efficiency degradation on the enteromorpha can be realized; the purpose of saving a water resource and a time are achieved; biological fuel gas can be prepared while the harm caused to an offshore oceanic environment by the enteromorpha is deceased; the obtaining method has favorable economic benefit and environmental benefit.

Description

technical field [0001] The invention belongs to the technical field of microbes and bioenergy, and in particular relates to a method for obtaining salt-tolerant microbial flora used for fermenting enteromorpha to prepare biogas and its application. Background technique [0002] Enteromorpha( Enteromorph ) is a large green algae belonging to Chlorophyceae and Ulvaceae. The common species include Enteromorpha enteromorpha, Enteromorpha flatus, Enteromorpha marginatus, Enteromorpha barbata, etc. Enteromorpha has tenacious vitality, high environmental adaptability, and fast reproduction speed. Therefore, its large-scale growth will have a strong inhibitory effect on the growth of other algae in the same sea area. In recent years, due to the aggravation of marine environmental pollution and eutrophication of seawater, the vicious reproduction of Enteromorpha has been caused. At the same time, Enteromorpha floats to the coast to rot and stink, not only causing great harm to the ...

AI Technical Summary

Problems solved by technology

Patents CN101418315A, CN 102876727A, and CN 104642530A provide a variety of methods for producing biogas using Enteromorpha as raw materials, but these methods need to use clean water to remove the salt in Enteromorpha or prepare fermentation broth with fresh water, because Enteromorpha belongs to the ocean The algae after harvesting Enteromorpha carry a large amount of sea salt, while the fermentation flora used in the previous technology is usually enriched from lake sludge, hot spring silt, animal manure, and the tolerance to sea salt is insufficient, resulting in the utilization of Enteromorpha fermented to produce biogas is difficult to achieve industrial production

It can be seen that in order to solve the problem that the current process of Enteromorpha fermentation to produce biogas cannot achieve the desired effect, the problem to be solved urgently is how to obtain a salt-tolerant and efficient Enteromorpha-degrading bacterial group