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Plant callus culture utensil

A technology of callus and culture vessel, which is applied in the direction of tissue cell/virus culture device, bioreactor/fermenter combination, specific-purpose bioreactor/fermenter, etc. Requirements, unfavorable plant callus culture, unstable cover and bottom installation, etc., to achieve the effect of solving the unstable cover and bottom, simple structure and convenient operation

Pending Publication Date: 2017-05-31
HUZHOU TEACHERS COLLEGE
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

Most of the existing culture vessels used for plant callus culture have unstable lids and bottoms and a certain risk of bacterial contamination. In order to solve these problems, some external materials are needed to stabilize the operation. cumbersome
On the other hand, the existing culture vessels also have the disadvantage of poor air permeability, which cannot meet the requirements for air circulation and moisture in the plant callus culture process, which is not conducive to plant callus culture.

Method used

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  • Plant callus culture utensil
  • Plant callus culture utensil
  • Plant callus culture utensil

Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0020] A plant callus culture vessel, comprising a culture vessel 1 and an upper cover 2, both of which are made of transparent materials. The petri dish 1 is seamlessly connected with the upper cover 2, and a detachable and recyclable connection belt 4 is set between the petri dish 1 and the upper cover 2 to fix it. Two mutually symmetrical The protruding point is provided with a corresponding protruding point at the edge of the upper surface of the upper cover 2 at the same time, and the protruding point is used for the connection belt 4 to carry out snap-type fixed connection of the culture dish 1 and the upper cover 2 . At the same time, a cylindrical ventilation cavity layer 3 is provided at the middle position on the upper surface of the upper cover 2, and the cylindrical ventilation cavity layer 3 includes a cover portion 32 with 2-4 small ventilation holes 34 and a connection with the culture dish. The bottom 31 of the upper cover 2 is injection-molded and provided wit...

Embodiment 2

[0025] A plant callus culture vessel, compared with Example 1, except that the structure of the cylindrical ventilation cavity layer 3 in the upper cover 2 of the culture vessel 1 or the selection of materials are partially changed, the rest are the same as those in Example 1. One said the same. The change is that the connection mode between the cover portion 32 containing the small air holes 34 of the cylindrical ventilation cavity layer 3 and the bottom 31 which is injection-molded with the upper cover 2 of the culture dish and provided with a gas-permeable membrane is different, wherein The material of the selected cover 34 needs to have a certain degree of elasticity. The cover 32 and the bottom 31 are connected by the elasticity of the material itself in a manner similar to snap buttons, and an inner concave point 36 is set at about half the height of the cover 32 as a fixed point. And the diameter of its cover 32 needs to be slightly greater than the diameter of the bott...

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Abstract

The invention relates to a plant callus culture utensil which comprises a petri dish and an upper cover, wherein the petri dish is in seamless gap connection with the upper cover; a detachable recyclable connecting strip for fixation is arranged between the petri dish and the upper cover; convex points in mutual symmetry are arranged between the petri dish and the upper cover to achieve buckle type fixed connection of the petri dish and the upper cover; a columnar ventilation cavity layer is arranged right in the middle of the upper surface of the upper cover; the columnar ventilation cavity layer is provided with a cover part with small vent holes and a bottom which is integrated with the upper cover of the petri dish in an injection molding manner and is provided with a ventilation membrane; and the cover part with the small vent holes can be opened to replace degreased cotton or the ventilation membrane inside the cavity layer. The plant callus culture utensil is capable of effectively solving the defect that the cover plate and the bottom of a conventional petri dish are not stable, and is simple to process, environmentally friendly and effective, convenient to operate, beneficial to sunshine radiation, capable of effectively preventing bacterium invasion to damage plant tissue and ensuring circulation of air and moisture, and beneficial to culture of plant callus tissue.

Description

technical field [0001] The invention relates to a plant tissue culture vessel, in particular to a plant callus culture vessel. Background technique [0002] At present, the materials of petri dishes are basically divided into two types: plastic and glass, generally consisting of a flat disc-shaped bottom and a cover, which can be used for plant materials, microbial culture, animal cell adherent culture and laboratory inoculation, Scribe, isolate bacteria, etc. Most of the existing culture vessels used for plant callus culture have unstable lids and bottoms and a certain risk of bacterial contamination. In order to solve these problems, some external materials are needed to stabilize the operation. cumbersome. On the other hand, the existing culture vessels still have the disadvantage of poor air permeability, which cannot meet the requirements for air circulation and moisture in the plant callus culture process, and are not conducive to plant callus culture. Contents of ...

Claims

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Application Information

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Patent Type & Authority Applications(China)
IPC IPC(8): C12M3/00C12M1/22C12M1/04
CPCC12M23/10C12M23/24
Inventor 杨志红严丹露
Owner HUZHOU TEACHERS COLLEGE
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